摘要
目的研究酒精对乙肝病毒复制和基因表达的影响,并探讨其发生机制。方法通过灌胃途径对实验组HBV转基因小鼠予以酒精干预,并在实验第6周末处死。同期设生理盐水组作为对照。定量测定血清HBV-DNA、HBsAg、HBeAg水平,肝组织切片行HBcAg免疫组化。检测血清HBV-DNA阳性查体者戒酒前后血清病毒载量水平。结果HBV转基因小鼠酒精组血清HBV-DNA载量、HBsAg水平、HBeAg阳性率高于对照组;转基因小鼠酒精组肝细胞HB-cAg免疫组化阳性率较对照组明显升高;轻度饮酒组戒酒前后病毒载量差异无统计学意义,中度饮酒组与重度饮酒组戒酒前后病毒载量差异均有统计学意义。结论酒精能促进体内HBV-DNA复制和基因表达。
Objective To study the influence of ethanol on HBV replication and gene expression and its possible mechanism. Methods HBV transgenic mice of experiment group were fed with ethanol through infusion. These mice were sacrificed at the end of the 6th week during the study, The other transgenic mice fed with physiological saline were taken as controls at the same stage during the study. The serum levels of HBV - DNA, HBsAg and HBeAg were measured quantitatively. Liver sections were dyed by HBcAg immunohistochemistry. The level of HBV - DNA was measured before and after stopping drinking. Results The level of serum HBV - DNA and HBeAg of ethanol group was higher than that of control group. The hepatocyte HBcAg positive rate of HBV transgenic mice fed with ethanol was higher than that of physiological saline - fed HBV transgenic mice. There was no difference in slightly drinking group,but there was significant difference in moderate and heavy drinking groups before and after stopping drinking. Conclusion Ethanol stimulates HBV -DNA replication and gene expression in vivo.
出处
《临床医学》
CAS
2007年第4期75-77,共3页
Clinical Medicine
基金
山东省卫生厅资助(1998CAICJB5)
