摘要
目的探讨青蒿琥酯在体外对乙型肝炎病毒复制及肝癌细胞株HepG2.2.15凋亡的影响。方法将不同浓度青蒿琥酯作用于转染乙型肝炎病毒全基因组DNA的肝癌细胞株HepG2.2.15,收集48 h上清,采用酶联免疫吸附实验检测上清中乙型肝炎病毒表面抗原(HBsAg)和e抗原(HBeAg),采用荧光定量PCR法检测HBV-DNA,流式细胞术检测细胞凋亡。结果青蒿琥酯对HBV复制具有抑制作用,随着浓度增加,对HBsAg和HBeAg的抑制率逐渐上升,细胞内HBV-DNA复制水平下降;青蒿琥酯可诱导肝癌细胞早期凋亡及导致细胞死亡,随浓度增加,HepG2.2.15细胞早期凋亡率及死亡率均增加。结论青蒿琥酯对HepG2.2.15细胞HBsAg和HBeAg的分泌及HBV-DNA复制具有抑制作用,并具有诱导HepG2.2.15细胞凋亡的作用。
Objective To investigate the effect of artesunate on hepatitis B virus（HBV） replication and the apoptosis in the hepatoma cell line HepG2.2.15.Methods The HepG2.2.15 cells were treated with different concentrations of artesunate for 48 hours.Hepatitis B surface antigen（HBsAg） and hepatitis B e antigen （HBeAg） in supernatant were detected by ELISA,HBV-DNA was decteted by fluorescence quantitative PCR,and HepG2.2.15 cell apoptosis was detected by Flow cytometry.Results Artesunate suppressed expressions of HBsAg and HBeAg,and inhibited the replication of HBV-DNA.Artesunate induced HepG2.2.15 cell early apoptosis and leaded to HepG2.2.15 cell death.With the artesunate concentration higher,the role was stronger.Conclusion Artesunate suppresses expressions of HBsAg,HBeAg and replication of HBV-DNA in HepG2.2.15 cells,and induces HepG2.2.15 cell apoptosis.Artesunate could be used to treat posthepatitic primary hepatic carcinoma.
出处
《胃肠病学和肝病学杂志》
CAS
2010年第1期71-74,共4页
Chinese Journal of Gastroenterology and Hepatology
