摘要
利用盐酸林可霉素在紫外区域无吸收峰这一特点，直接用紫外分光光度法在２７３±１ｎｍ波长处测定盐酸氟哌酸的含量；用甲醇分离两组分后，用微生物测定法测定盐酸林可霉素的效价。实验结果，盐酸氟哌酸的回收率为１００．２１％，ＣＶ＝０．３８％；盐酸林可霉素的回收率为９９．２％，ＣＶ＝０．１８％。
Ultra Violet Spectrophotometry has been applied successfully to the determination of Norloxacin hydrochlorid, depending on Lincomycin hydrochlorid having no absorption spectrum in the rang 200 to 400 nm. The absorbance was measured at the maximun at 273nm. The biological assay of antibiotics of Lincomycin hydrochlorid was accomplished after the separation from Norfloxacin hydrochlorid with methanl, the recovery of Norloxacin hydrochlorid is 100.2%, CV=0.38%; the recovery of Lincomycin hydrochlorid is 99.2%, CV=0.18%
出处
《中国兽药杂志》
北大核心
1997年第1期27-28,共2页
Chinese Journal of Veterinary Drug
关键词
盐酸林可霉素
盐酸氟哌酸
测定
鳗菌宁1号
Norfloxacin hydrochlorid
Lincomycin hydrochlorid
Ultra Violet Spectrophotometry
The biological assay of antibiotics.
