摘要
目的:观察40例金属烤瓷冠修复患者变色牙龈的超微结构,测定其中超氧化物歧化酶(SOD)和还原型谷胱甘肽(GSH)的活性,探讨牙龈变色的机制。方法:以健康牙龈组织作为空白对照,透射电镜观察牙龈组织的超微结构;X线能级分析牙龈组织的金属能谱;采用羟胺法测定SOD值,改良双甲基苯甲酸定量法测定GSH值,数据经SPSS10．0软件包进行t检验及单因素方差分析。结果:(1)金属烤瓷修复后的变色牙龈组织结构异常;(2)X线能级分析变色牙龈组织未见镍、铬元素波谱;(3)金属烤瓷修复后的变色牙龈组织与正常牙龈中SOD、GSH活性有显著差异(P<0．05),且修复后6-18个月与其他时间段差异显著(P<0．05)。结论:金属烤瓷修复后的变色牙龈组织结构改变,牙龈组织中自由基清除剂活性不断变化,其代谢失衡和细胞凋亡可引发结构和颜色改变。
PURPOSE： This study was designed to study the discolored gingiva adjacent to porcelain fused to metal （PFM） crowns in terms of ultrastructure , SOD and GSH activities in 40 cases. METHODS： The discolored gingival ultrastructures were observed and metal X-ray energy level was analyzed;The activities of SOD and GSH were measured and compared with normal control by student＇s t test and one-way ANOVA with SPSS10.0 software package. RESULTS： The discolored gingival ultrastructure had changes compared with the normal gingiva. Nickel and chromium were not found in the particles through X-ray energy machine within the discolored gingiva adjacent to PFM crown. The activities of SOD and GSH in discolored gingiva were significantly different from control （P〈0.05） and the values at 6 to 18 months were significantly different from those at other times. CONCLUSION： The ultrastructure underwent changes in discolored gingiva after PFM restoration; the activity of SOD and GSH in discolored gingiva changed to result in apoptosis, and discoloration.
出处
《上海口腔医学》
CAS
CSCD
2007年第2期176-179,共4页
Shanghai Journal of Stomatology
