摘要
以啤酒酵母粉为原料,综合考虑提取率、蛋白质含量和多糖含量3个指标,在单因素实验的基础上,采用正交实验,得出碱-酶法提取废啤酒酵母中β-(1,3)-D-葡聚糖的理想工艺条件:酵母粉经过水洗,脱色后,按照150mL∶10g的比例加入3%的NaOH溶液,在80℃水浴条件下水解2h,离心,洗涤,调整pH至8.5～9.0,再加入600U/g碱性蛋白酶(以干酵母粉计),在55℃下水解24h,离心,沉淀,干燥得到成品。β-(1,3)-D-葡聚糖的提取率为13.8%,产品多糖含量85.2%、蛋白质含量1.2%、水分9.2%,产品为乳白色粉状固体,色度为L值83.07、a值2.12、b值8.86。
To optimize the extraction technology of β-（1, 3）-D-glucan from beer waste yeast with alkalienzymatic method, orthogonal test was applied based on the single-factor experiments with dry yeast powder as raw material and extract rate of β-（1, 3）-D-glucan, protein content and polysaccharid content were taken into account in this paper. The optimal extraction condition was： dry beer yeast powder decoloured was washed by water, then, it was added to 3% sodium hydroxide according to the ratio of 1 ： 15（W/V）, the mixture was hydrolyzed for 2.0 hours at 80℃ and centrifuged, washed, then 600U/g alkalescent protease was added to the water solvent after the pH value of solution was modified to 8.5-9.0, at last, the mixture was hydrolyzed for 24hours at 55℃, after centrifuged and washed, the product was attained after the sediment was dried. Yield ratio of the final product was 13.8%, and the content of polysaccharide, protein and moisture was 85.2%, 1.2%, 9.2, respectively.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2007年第4期145-149,共5页
Food and Fermentation Industries
