摘要
目的研究邻苯二甲酸酯类对大鼠睾丸毒性作用的分子机制。方法运用RT-PCR方法观察邻苯二甲酸二丁酯(DBP)对大鼠子代不同发育阶段睾丸的苗勒氏管抑制物质(MIS)、雄激素结合蛋白(ABP)和抑制素(inhibin)表达水平的影响,并采用ELISA法检测DBP染毒后大鼠睾酮水平的变化情况。结果与对照组比较,各DBP染毒组大鼠睾丸中MIS表达差异无统计学意义,血清总睾酮水平差异无统计学意义,但高剂量DBP(1000 mg/kg)组睾丸支持细胞中ABP和抑制素(inhibin)的mRNA表达量显著减少,且与睾丸损害呈时间-效应关系。结论环境内分泌干扰物DBP可干扰大鼠发育过程中睾丸支持细胞ABP和inhibin的表达,其对性腺发育的影响可能是DBP雄性生殖毒性的主要作用机制之一。
Objective To study the role of hypothalamus - pituitary - gond axis played on the mechanism of male toxicity of di - n - butyl phthalate（DBP）. Methods The testosterone concentration and relative express/on levels of MIS, ABP and inhibin in different developmental stage of rats＇ testes were measured by methods of ELISA and RT - PCR. Results There was no significant difference in MIS expression and testosterone levels in DBP- treated groups while comparing with the control group. However, 1 000 mg/kg DBP can significantly decrease the ABP and inhibin expression levels of rats＇ testes in a time - dependent manner. Conclusion DBP could decrease the expression of testosterone binding ABP and inhibin, but not MIS and testosterone, suggesting that antiadrogenic activity by disturbing the function of hypothalamus- pituitary- gonad axis might be one of the reasons of male reproductive and developmental toxicity induced by DBP.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2007年第5期631-633,共3页
Chinese Journal of Public Health
基金
国家自然科学基金(30500397)
上海市卫生局青年基金资助(054Y32)
关键词
邻苯二甲酸酯类
苗勒氏管抑制物质
雄激素结合蛋白
di - n - butyl phthalates（DBP）
miillerian inhibiting substance（MIS）
androgen - binding protein （ABP）
