摘要
目的研究芒果苷诱导慢粒白血病细胞系K562细胞凋亡的机制。方法采用RT—PCR检测芒果苷（254-200μmol／L）处理（24、48、72、96h）后K562细胞中bcl-2 mRNA、bax mRNA、survivin mRNA基因表达变化；采用Western blotting方法检测K562细胞BCR／ABL融合蛋白质P210水平。结果芒果苷作用K562细胞后，P210蛋白质水平下调，并呈时间及剂量依赖性，bax基因表达显著上调，bcl-2基因表达轻度下调，survivin mRNA基因表达下调。结论芒果苷诱导K562细胞凋亡的机制可能是通过下调BCR／ABL融合蛋白质P210、bcl-2和survivin mRNA基因表达及上调bax基因表达来实现的。
Objective To study the apoptosis mechanism of K562 cell lines induced by mangiferin. Methods The mRNA expression levels of apoptosis-related genes including bcl-2, bax, survivin of K562 cells treated by mangiferin （25-200μmol/L） for 24, 48, 72, and 96 h were determined by RT-PCR; the BCR/ABL protein P210 level was detected by Western blotting. Results Mangiferin up-regulated bax gene of K562 cells significantly and down-regulated bcl-2 gene slightly, resulting in an enhancement of the ratio of bax/bcl-2. Mangiferin down-regulated the expression levels of P210 in K562 cells in a time- and concentration-dependent manner and so is the expression level of survivin mRNA in K562 cells. Conclusion The mechanism of mangiferin-induced apoptosis in K562 leukemic cells might be involved in up-regulating the gene expression of bax and down-regulating the mRNA expression of BCR/ABL protein P210, bcl-2, and survivin.
出处
《中草药》
CAS
CSCD
北大核心
2007年第5期715-719,共5页
Chinese Traditional and Herbal Drugs
