摘要
分子体外定向进化是改造酶分子的新策略,它主要通过体外模拟自然进化机制,利用基因随机突变、重组和定向筛选技术,使进化过程朝着人们需要的方向发展。简要介绍了酶分子体外定向进化的发展历史,详细介绍了突变文库构建和筛选方法的最新研究进展及应用情况。
Directed molecular evolution in vitro is a new powerful stragy for engineering enzyme. It mainly simulated the process of natural evolution in the laboratory （mutation,recombination and selection）and evolve enzyme as desired.This review provide information about the history of directed molecular evolution in vitro and the recent progress of techniques for generation gene libraries and high-throughput screening methods,and provide also information about their applications.
出处
《生物技术通报》
CAS
CSCD
2007年第2期63-66,共4页
Biotechnology Bulletin
