摘要
目的:研究三七总皂苷(PNS)对人脐静脉内皮细胞(HUVEC)的增殖作用。方法:采用体外培养HUVEC的方法,通过XTT法以及细胞计数法观察对细胞的增殖作用;细胞侵入实验观察细胞迁移的能力。结果:PNS对HUVEC有促增殖作用,XTT实验中100μg/ml浓度增殖率为(33.58±2.41)%;在细胞计数实验100μg/ml浓度增殖率为(94.15±10.96)%;在细胞侵入实验中100μg/ml浓度侵入率为(42.64±9.50)%。结论:PNS能够促进HUVEC的增殖与侵入。
Objective： To investigate the proliferation efficiency of total saponins of Panax notoginseng （PNS） on human umbilical vein endothelial cell （HUVEC）. Methods： HUVEC were incubated in vitro. The extract was identified to stimulate the proliferation of HUVEC by xTr assay and microscopic cell counting; and the numbers of invaded cells and the mean tube length were significantly increased in both treatment groups; Results： The HUVEC cells increased proliferation in the response to stimulation of PNS. In the XTT assay, after being incubated with PNS 100μg/ml, the increasing rate was（33.58 ± 2.41 ）% ; In the cell counting assay, the ceils were raised by（94.15 ± 10.96）% at the concentration of PNS 100μg/ml; And in the invasion assay, the PNS could induce the cells＇invasion with the concentration of 0.10μg/ ml, 10μg/ml, 100μg/ml, and there was（42.64 ±9.50）% enhanced invasion at the concentration of 100μg/ml. Conclusion： PNS can promote the proliferation and invasion of HUVEC cells.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2007年第2期28-30,共3页
Pharmacology and Clinics of Chinese Materia Medica
基金
澳门科技发展基金资助项目(078/2005/A2)
关键词
三七总皂苷(PNS)
人脐静脉内皮细胞(HUVEC)
增殖
total saponins of Panax notoginseng （PNS）
human umbilical vein endothelial cell （HUVEC）
proliferation
