摘要
甘氨酸甜菜碱、脯氨酸及葫芦巴碱的含量与葡萄的抗逆性能密切相关，应用离子交换树脂提取葡萄叶中的这三种物质，采用Suger-pak 1（waters）色谱柱，柱温：85℃；样品测定时间：70min；进样体积：100μL；流速：0．6mL／min；检测波长：195nm；流动相：50mg／L Ca—EDTA的HPLC同时分析测定这三种物质，甘氨酸甜菜碱、脯氨酸及葫芦巴碱线性相关系数分别为0．9999，0．9994及0．9994；保留时间分别为20．28，25．66及30．65min；检出限分别为1．35，2．56及1．02ng／mL，回收率为93％～96％。此方法分离效果好，稳定，精确。
The concentration of glyclne betaine,proline and trigonelline in grape leaves is close relative to the ability of grapes＇ anti-stress. They were extracted by ion-exchange resin and tested by HPLC. This paper developed the method of HPLC, which included column temperature ： 85 ℃, run time： 70 min, inject volume ： 100 μL, flow rate： 0.6 mL/min, k： 195 um,colunm：suger-pak 1 （waters） ,mobile phase：50 mg/L Ca-EDTA. The method is stable. The relationship coefficients of glycine betaine, proline and trigonelline were respectively 0. 9999,0. 9994 and 0. 9994, the retention time were 20.28,25.66 and 30.65 min, and detection limits were 1.35,2.56 and 1.02 ng/mL by the method of HPLC. Recoveries were 93% -96%.
出处
《天然产物研究与开发》
CAS
CSCD
2007年第2期313-315,共3页
Natural Product Research and Development
