摘要
建立以唑类有机化合物（4-氨基-4H-1,2,4,-三唑）修饰玻璃电极,得到聚唑类有机合物膜修饰电极,并以该电极对多巴胺（DA）和黄嘌呤（XN）进行同时分析测定。结果表明,在0.2 mol·L^-1的磷酸盐缓冲液（p H=5.0）中,DA和XN在聚唑类有机化合物修饰的电极上具有比较好的电化学行为。多巴胺和黄嘌呤分别5×10^-5~5×10^-9mol·L^-1和1×10^-4~1×10-7mol·L^-1浓度范围内有较好的电化学响应,DA和XN检出限分别为5×10^-10mol·L^-1和1×10^-8mol·L^-1,该修饰电极具有较高的灵敏度、重现性和稳定性。该方法可用于人体的尿液中两种化合物的同时测定分析,回收率分别为94.7%~99.3%与93.6%~95.3%.
The glassy carbon electrode（GCE） was modified by an ultrathin electro polymerized film of organics （4-amino-4H-1,2, 4,-triazole） in this paper. The electrochemical behavior of the modified electrode was eharacterizedusingeleetrochemieal impedance method, differential pulse voltammetry and cyclic voltammetry. The results showed that dopamine and xanthine in O. 20M phosphate buffer solution（pH5.0）had excellent electrochemical behavioratthis modified electrode. Dopamine and xanthine had good electrochemical response in the eoncentration range of 5 × 10^-5 -5 × 10^-9 mol· L^-1 and 1 × 10^-4 - 1 × 10^-7 mol· L^-1 respectively. Dopamine and xanthine detection limit was 5×10^-10 mol· L^-1 and 1 × 10^-8mol· L^-1, respectively. The method was used to analysisof human urine samples,the recoveries were 94. 7% - 99. 3% and 93.6% - 95.3%.
出处
《化学研究与应用》
CAS
CSCD
北大核心
2015年第8期1123-1127,共5页
Chemical Research and Application
基金
国家自然科学基金项目(21465020)资助
江西省教学改革课题(JXJG-14-16-13)资助
