摘要
目的研究β3-肾上腺素能受体基因的Trp64Arg变异与UCP2基因Ala55Val变异对儿童单纯性肥胖的影响是否存在交互作用,为儿童单纯性肥胖的防治提供理论依据。方法用聚合酶链反应—限制性片段长度多态性方法(PCR-RFLP)检测UCP2基因Ala55Val及β3-AR基因Trp64Arg的多态性,比较病例组与对照组各基因型的发生频率以及不同基因型之间BMI、血液生化指标的差异。结果β3-AR的变异频率在单纯性肥胖组和对照组差异有统计学意义(P=0.038),UCP2基因的变异频率在2组之间差异也有统计学意义(P=0.045)。当只有单一的UCP2或β3-AR变异基因型时,病例组与对照组的频率分布差异无统计学意义;但当UCP2和β3-AR基因同时发生变异时,病例组的变异基因频率明显高于正常组(OR=4.002,95%可信区间为1.636～9.884)。结论UCP2基因Ala55Val变异与β3-AR基因Trp64Arg变异同时发生时,会明显增加儿童单纯性肥胖症发生的危险性。
Objective To investigate the interaction effect of uncoupling protein 2（UCP2） gone Ala55Val variation and β3-adrenerglc receptor（β3- AR） geue Trp64Arg variation on children＇s simple obesity, and to provide theoretical evidence for the prevention. Methods PCR-RFLP was used to detect the UCP2 gone Ala55Val variation and β3-AR gone Trp64Arg variation, The allele frequency distributions among case group and control group, and the difference of BMI and biochemical index between genotypes, were analyzed. Results The frequency of β3-AR gene Trp64Arg variation in simple obesity children was significantly different from that in control group（ P = 0.038）. The frequency of UCP2 gene Ala55Val variation in simple obesity children was significantly different from that in control subjects （ P = 0.045）. When there was only UCP2gene or β3-AR gene mutation, the frequency of gone mutation in simple obesity children was not significantly different from that in control group. But when there were simultaneously two gene mutations, the frequency of gene mutations was higher in simple obesity children than in control group （ OR = 4.002, 95 % CI ranged 1.636 - 9.884）. Conclusion The accumulated effect of the two microgenes increase the risk of the occurrence of children＇s simple obesity.
出处
《中国学校卫生》
CAS
北大核心
2007年第3期249-252,共4页
Chinese Journal of School Health
基金
江苏省高校自然科学研究项目(编号:02KJD330001)
江苏省"333工程"培养资金资助项目
关键词
受体
肾上腺索能β3
多态性
单核苷酸
肥胖症
儿童
Receptors, adrenergic
beta-3
Polymorphism, single nucleotide
Obesity
Child
