摘要
斜带石斑鱼和赤点石斑鱼为具有重要经济价值的海水鱼类。对这两种石斑鱼进行了随机扩增多态DNA(RAPD)和线粒体细胞色素b(Cyt b)基因序列变异分析。计算多态位点百分率、基因多样性和香农信息指数等遗传参数,以此评估种内遗传变异水平;通过统计变异位点、平均核苷酸差异数、核苷酸多样性以及种间平均每位点核苷酸替代数进行基因序列变异分析,并构建UPGMA系统树。结果表明:斜带石斑鱼的遗传多样性水平高于赤点石斑鱼,这可能与它们在种内特定遗传结构、分布范围大小、自然资源状况的差异有关;斜带石斑鱼和赤点石斑鱼之间检测到的种特异RAPD条带以及基因序列的变异,可作为种间分子鉴定标记。
Epinephelus coioides and E. akaara are commercially important marine fish species. The genetic variation of those two species were analyzed by random amplified polymorphic DNA （RAPD） and mitochondrial cytochrome b （Cyt b） sequencing techniques Genetic diversity was evaluated by three parameters including percentage of polymorphic loci, gene diversity and Shannon＇s information index. In addition, variable sites, nucleotide diversity, average number of nucleotide differences and average number of nucleotide substitutions per site were calculated to analyze sequence difference, and phylogenetic trees were constructed by the UPGMA method. The results showed that E. coioides had higher genetic diversity than E. akaara, possibly related to different genetic structure, geographical distribution range and the condition of natural resource between species. The species-specific RAPD band and gene sequence difference were detected between E. coioides and E. akaara, which could be served as molecular markers for species identification.
出处
《中山大学学报（自然科学版）》
CAS
CSCD
北大核心
2007年第1期75-80,共6页
Acta Scientiarum Naturalium Universitatis Sunyatseni
基金
国家海洋863计划资助项目(2001AA621010
2003AA603011)
中国博士后科学基金资助项目(2004036521)
中国水产科学院水产种质资源与养殖技术重点开放实验室开放基金资助项目(2004A006)
