摘要
从林区土壤中分离能够分解纤维素的细菌菌株共15株,采用刚果红染色的初筛平板获取透明圈较明显的菌株8株。在此基础上,进行液体培养并测定酶活,得到2株酶活较高的菌株,分别编号为X18和X10-1-2。这两株菌均为G(+)、形态杆状、可产芽孢、好氧,初步鉴定为芽孢杆菌属的两个不同种。
The 15 strains of bacteria producing cellulase were isolated from soil. Eight colonies surrounded by some distinct zones of clearing in cellulose - congo red agar medium from the 15 strains above were obtained. By measuring the enzyme activity of liquid culture filtrates, two strains （termed X18 and X10 - 1 -2, respectively） with higher cellulase activity were obtained. They are gram positive belong to the Bacillus genus.
出处
《黑龙江大学自然科学学报》
CAS
北大核心
2007年第2期211-213,218,共4页
Journal of Natural Science of Heilongjiang University
基金
国家自然科学基金资助项目(50178021)
关键词
纤维素酶
细菌
筛选
cellulase
bacteria
screening , endospore forming and aerobic bacteria. They all
