摘要
以凉山半细毛羊、山谷型藏绵羊为研究对象,以微量全血培养法制备染色体,经对大量分裂相的分析结果表明:凉山半细毛羊、山谷型藏绵羊二倍体细胞核型均为公羊2n=54,XY,母羊2n=54,XX;两品种羊染色体数目变异频率分别为33.00%,32.25%;结构变异频率分别为12.00%,17.60%,染色体结构变异主要表现为染色体断裂、染色单体臂断裂及着丝粒处变异等;将染色体或染色单体断裂位点与Ag-NORs特殊位点作比较发现部分位点间存在一一对应情况;同源染色体不等长现象在两品种羊中均有发现,且凉山半细毛羊较山谷型藏绵羊明显。
Four Liangshan Semifine-wool sheep and four Tibetan sheep were used to compare the karyotypes, which were employed on slides obtained from the peripheral blood cell culture. The results showed that the karyotype of ram was 54, XY; and that of ewe was 54, XX. In the two breeds,the frequency of chromosomal structural change were 12.00% and 17.60%, and the frequency of chromosomal numerical change were 33.00 % and 32.25 % ;the type of the chromosomal structural change includ chromosome break, chromatid break and centric change; Some break points of chromosomal structural change were correspondence with the loci of specific Ag-NORs; Some homologues had the different length in the two breeds, and this phenomenon in Liangshan Semifine-wool sheep was more significant than in Tibetan sheep.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2007年第4期400-406,共7页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
德国联邦科技与教育部(BMBF)资助(CHN00316)
上海市重点学科建设项目资助(Y1101)
