摘要
研究了用三氯乙酸(TCA)脱除蛋白质提取钝顶螺旋藻(Spirulina platensis)多糖的工艺,并以提取率、样品糖含量与蛋白含量为指标,应用正交实验设计优化了提取液pH值、提取温度及提取时间3个因素。结果表明,在提取液pH值为12、提取温度为90℃及提取时间为8h的最佳条件下,所得螺旋藻多糖为白色粉末,提取率为3.10%,糖质量分数为81.79%,蛋白质量分数为0.57%。
The extraction technology of polysaccharides from Spirulina platensis by removing protein with TCA was studied in this article, the orthogonal design was employed to test the effects of the three factors including pH of water, temperature of extraction and time of extraction on the extraction rate, total carbohydrate contents and protein contents of polysaccharides from S. platensis and optimize the three factors. The final result indicated that the optimum pH of water, temperature and time of extraction were 12, 90℃ and 8h, under this optimum condition the fractim of polysaccharide gained from S. platensis was a white powder, the extraction rate, the fraction of carbohydrate contents and fraction of protein contents were 3. 10%, 81. 79% and 0.57%.
出处
《海洋科学》
CAS
CSCD
北大核心
2007年第4期42-47,共6页
Marine Sciences
