摘要
目的探讨络活喜(amlodipine)对自发性高血压大鼠(spontaneously hypertensive rats,SHR)血管壁重塑生物学机制。方法将10周龄雄性24只SHR随机分成3组,每组8只。对照组(SHRC)不作治疗,并与同周龄Wistar鼠组(n=8)作比较。生理盐水组(SHRNS),络活喜组(SHRA)分别经生理盐水、络活喜治疗8周后光镜观察血管几何形态,采用原位杂交及半定量RT-PCR方法检测ET-1(endothelin-1)、MMP-2(matrix metal-loproteinase-2)表达水平。结果对照组,生理盐水组血压明显增高,管腔增大,管壁明显增厚(P<0.05),尤以血管中层平滑肌细胞(vascular smooth cell,VSMC)肥大为主(P<0.01);ET-1,MMP-2表达显著增加(P<0.05)。治疗8周后,络活喜组血压明显降低,管壁变薄;ET-1、MMP-2表达减少(P<0.05)。结论络活喜可能通过抑制血管壁ET-1与MMP-2表达,遏制自发性高血压大鼠血管壁重塑。
Objective To investigate the effects and biological mechanism of amlodipine on vascular remodeling in sponta- neous hypertensive rats（SHR）. Methods Twenty- four male SHR rats （10 weeks old） were randomly divided into three groups： Group 1： control group （SHRC）, which were fed without any treatment served as blank control to compare with the same aged Wistar rats; Group 2： sodium chloride group （SHRNs）, gavaged with 0.9% sodium chloride （10 ml/kg·d）; Group 3： amlodipine group （SHRA）, fed with amlodipine （30mg/kg·d）. At the end of the study, vascular geometry was determined by optic microscope; the expression of endothelin - 1 （ET - 1 ） and matrix metaloproteinase - 2 （MMP - 2） were detected with situ hybridization and half quantitative reverse transcription- polymerase chain reaction （RT- PCR）. Results Compared with normal Wistar rats, the blood pressure, the out diameter or inner diameter and the wall thickness of SHRc and SHRNS were increased obviously （ P 〈 0.05）, and hypertrophic changes in vascular smooth muscular cell （VSMC） of the aorta were obvious （ P 〈 0.01）. Moreover, the expression of ET- 1 and MMP- 2 was upregulated （ P 〈 0.05）. After 8 weeks of treatment with amlodipine, the blood pressure decreased obviously （ P 〈 0.01）, the wall thickness of the aorta became thinner, the expression of ET - 1 and MMP - 2 decreased in SHRA（ P 〈 0.01）. Conclusions Amlodipine may restrain vascular remodeling by clown - regulating expression of ET - 1 and MMP - 2 in blood vessel in SHR rats.
出处
《心脑血管病防治》
2007年第2期92-94,共3页
CARDIO-CEREBROVASCULAR DISEASE PREVENTION AND TREATMENT
关键词
自发性高血压
血管壁重塑
络活喜
大鼠
Spontaneous hypertensive rats
Vascular remodeling
Amlodipine
Rabbit
