摘要
目的:检测急性发作期哮喘患儿外周血辅助性T淋巴细胞(Th)亚群和血浆总IgE水平,并对二者的相关性进行分析。方法:以流式细胞术检测哮喘急性发作期患儿(30例)和对照组健康儿童(30例)的外周血辅助性T淋巴细胞亚群,并采用化学发光法检测其血浆总IgE水平。结果:(1)与对照组比较,急性发作期哮喘患儿Th1亚群百分比(14.7%±3.3%)和Th1/Th2比值(0.904±0.455)均显著降低(P<0.05,P<0.01),而Th2亚群百分比(14.7%±3.3%)显著升高(P<0.05)。(2)急性发作期哮喘患儿血浆总IgE水平为272.2±129.7,明显高于对照组(46.8±28.5),(P<0.01)。(3)急性发作期哮喘患儿Th2亚群与血浆总IgE水平呈明显正相关(!=0.920,P<0.01);同时,其Th1/Th2比值与血浆总IgE水平呈明显负相关(γ=-0.832,P<0.05)。结论:急性发作期哮喘患儿外周血辅助性T淋巴细胞处于明显失衡状态,表现为Th1亚群百分比和Th1/Th2比值明显降低、Th2亚群百分比明显升高。同时,辅助性T淋巴细胞亚群状态与其血浆总IgE水平密切相关。
Objective：To investigate T helper lymphocyte subpopulation in peripheral blood and serum IgE level in asthma children during acute attack,and to analyse the relationship between them.Methods.T helper lymphocyte subpopulations in peripheral blood in asthma children during acute attack （n=30）and the control group（healthy ehildren,n=30）and serum IgE were detected by floweytometry and electrochemical luminescence assay.Results： （1）Compared with the healthy children,the percentages of Th1 subpopulation （14.7%±3.3%）and the ratio of the Th1/Th2 （0.904±0.455）in asthmatic children group during acute attack was significantly deereased（P〈0.05,P〈0.01） ;and the percentage of Th2 subpopulation （1.46%±0.35%）was significantly higher than that of the healthy children （P〈0.05）. （2）The level of serum IgE was 272.2±129.7,whieh was significantly higher than that of the healthy children （46.8±28.5,P〈0.01）.（3）There was significant positive correlation between Th2 subpopulation and serum IgE level in asthma children during acute attack （γ=0.920,P〈0.01）. Meanwhile,negative correlation was found between ratio of Th1/Th2 and serum IgE level （γ=-0.832,P〈0.05）. Conclusion：T helper lymphoeytes is significantly out of balance in asthma children during acute attack, characterized by increase of Th2 subpopulation,deerease of Thl subpopulation and lower ratio of Th1/Th2 in peripheral blood.Furthermore,the state of T helper subpopulations is significantly correlated with serum IgE level.
出处
《中日友好医院学报》
2007年第2期70-72,共3页
Journal of China-Japan Friendship Hospital
