摘要
目的探讨缺氧对体外培养成骨细胞增殖及凋亡的影响。方法应用MTT法、丫啶橙检测缺氧对体外培养成骨细胞的增殖及凋亡的影响。结果缺氧抑制了成骨细胞的增殖,且提高了成骨细胞的凋亡率,上述改变随着缺氧时间的延长而更加明显。结论缺氧具有抑制体外培养成骨细胞增殖,促进成骨细胞凋亡的作用。
Objective To study the effect of hypoxia on osteoblast proliferation and apoptosis. Methods MTT and nucleic acidbound dye acridine orange were used for the observation of the proiferation and apoptosis of cultured osteoblasts in vitro. Results It was found that hypoxia had an effect on restraining the proliferation and decreasing the apoptosis of cultured osteoblasts. Conclusion Hypoxia can not only inhibit the proliferation of Osteoblasts but also facilitate their apoptosis.
出处
《临床军医杂志》
CAS
2007年第2期171-173,共3页
Clinical Journal of Medical Officers
基金
全军"十五"规划重点课题(01Z008)
