摘要
采用免疫组化的方法检测110例维吾尔族宫颈癌和45例维族正常宫颈组织中p53基因第四外显子第72位密码子Arg/Pro(p53 Arg72Pro)3种基因型Arg/Arg、Pro/Arg、Pro/Pro型中Ki-67、Bax蛋白的表达。初步探讨p53Arg72Pro不同基因型在新疆维吾尔族人群宫颈癌发生机制中的相关作用。结果发现在维吾尔族宫颈癌p53Arg72Pro三种基因型中Arg/Arg型的细胞增殖指数最高62.990±15.0706,而Pro/Pro型的Bax蛋白阳性表达率最高66.67%,其表达差异有统计学意义(P<0.05)。结果表明p53 Arg72Pro 3种基因型在抑制细胞增殖、诱导细胞凋亡方面可能存在差异,p53 Arg72Pro多态性可能通过不同的机制引起肿瘤的发生。
In order to investigate the carcinogenic mechanism of the different genotypes of p53 exon 4 codon 72 of cervical carcinoma in Uigur women, immunohistochemical staining was used to detect the expression of Ki-67, Bax protein of the 110 patients and 45 controls. The results showed that the proliferation index of the homozygous arginine was 62. 990 ＋ 15.0706 which was the highest in three genotypes; whereas the ratio of the expression of Bax protein in homozygous proline was 66.67%, which was higher than the other genotypes, In statistical analysis there were the significant differences in the expression of Ki-67, Bax protein between three genotypes（ P 〈 0.05）. We assumed that the functions of suppressing cellular proliferation and promoting cellular apoptosis have distinctions in different genotypes, and the two genotypes may have the different carcinogenic mechanism to develop cervical carcinoma.
出处
《石河子大学学报（自然科学版）》
CAS
2007年第1期60-64,共5页
Journal of Shihezi University(Natural Science)
基金
国家自然科学基金资助项目(30560046)
