摘要
目的:探讨K i-67表达、细胞凋亡与宫颈癌发生、发展之间的关系。方法:应用免疫组化SP法检测K i-67表达及TUNEL法检测凋亡细胞标记指数(AI)。结果:从正常宫颈-C IN各级-宫颈癌,K i-67阳性细胞标记指数逐渐升高,而凋亡指数/K i-67阳性指数的比率呈逐渐下降趋势,且C IN各组与宫颈癌相比差异有显著性(P<0.01,P<0.05)。结论:K i-67的表达与宫颈癌的发生、发展有关,与凋亡标记结合应用,可用于判断宫颈病变的预后并指导临床治疗。
Objective： To study the relationship between expression of Ki 67, cell apoptosis in cervical carcinogenesis. Methods： The expressions of Ki - 67 were tested by immunohistochemistry assay and the apoptotic labeling indices were deteeted by TdT mediated dUTP -biotin nick and labeling method （TUNEL） in normal cervical epithelia , intraepithelia neoplasia and squamous cervical carcinoma tissue. Results： Ki-67 labeling indiees increased gradually and the apoptotic labeling indices/Ki -67 labeling indices decreased gradually from normal cervical epithelia, intraepithelia neoplasia to squamous cervical carcinoma tissue （P 〈 0. 01, P 〈 0. 05 ） . Conclusion： The expressions of Ki - 67 and the apoptosis labeling indices are in correlation with the developing course of cervical squamous carcinoma premalignancy, they are significant in clinical treatment and prediction of cervix lesion.
出处
《中国妇幼保健》
CAS
北大核心
2005年第21期2766-2767,共2页
Maternal and Child Health Care of China
关键词
KI-67
凋亡
宫颈癌
Ki - 67
Apoptosis
Cervical carcinoma
