摘要
海马内移植内嗅皮层的大鼠脑经高浓度戊二醛固定液灌流固定和合理修切以暴露移植物，并电泳１０％辣根过氧化酶（ＨＲＰ）于２ｍｍ×２ｍｍ×１．５ｍｍ大小的移植物后，光镜下可见密集的ＨＲＰ顺行标记轴突严格位于海马ＣＡ１、ＣＡ３区腔隙分子层和省回分子层，呈现出与Ｔｈｙ—１免疫组织化学显示移植内嗅皮层的轴突投射完全相同的分布模式。在电镜水平上可见组织结构保存极佳的ＨＲＰ标记末梢和非标诏树突干或树突小棘形成的突触。结果表明：ＨＲＰ电泳于固定后脑组织更易被控制于特定的区域，从而避免在体注射ＨＲＰ因易于殃及或被动扩散至相邻区域所引起的结果误导，也可以获得比免疫组化方法显示突触联系更为优良的电镜图象。
Intrahippocampal. transplanted entorhinal cortex (EC) in adult rats were perfused with a fixative containing high concentration of glutaraldehyde (1.5%), followed by the electrophoresis of 10% horseradish peroxidase (HRP) into the properly exposed transplant of a size about 2mmｘ2mmｘ1.5mm. It was observed in the light microscopy that dense HRP orthograde labeled axons were sharply restricted within the lacunosum molecular layer of CA1, CA3 and the outer molecular layer of dentate gyrus (DG) with the same distribution pattern of the transplanted EC axons as that stained by Thy-1 immunohistochemistry. At electronmicroscopical level the well-preserved ultrastructral HRP-labeled axon terminal profiles were observed to form synapses with the HRP-unlabeled dendritic shafts or spines. The results indicate that the electrophoresis of HRP into the prefixed brain tissue can more easily get access to desired area than filling in vivo where the passive diffusion or dislocation of HRP into the tissue adjacent to designed area usually occurs and misled results are induced, and keeps the ultrastructure of synaptic connections much better than immunoelectronmicroscopy.
出处
《神经科学》
SCIE
CAS
1996年第1期5-8,共4页
Chinese Journal of Neuroscience
基金
国家自然科学基金
国家科委中国科学院匹配经费资助
关键词
脑组织
辣根过氧化酶
轴突示踪
光镜
电镜
horseradish peroxidase
axonal trancing
light microscopy
electron microscopy
