摘要
目的建立阿替洛尔片中阿替洛尔的含量测定方法。方法采用HPLC法，色谱柱为Dinamonsil-C18柱，以甲醇-水（550：470）1000mL，加庚烷磺酸钠960mg与无水醋酸钠82mg使溶解，加冰醋酸0．57mL为流动相；流速为1．0mL·min^-1；检测波长为276nm,柱温为28℃。结果阿替洛尔进样量在0．1800～1．8000μg呈良好线性关系；其回归方程为Y=0．0781X（r=0．9999）；平均回收率为97．08％；RSD为1．33％（n=9）。结论本方法操作简单，准确，可用于测定阿替洛尔片的含量。
Objective To determine the contents of atenolol in atenolol tablets. Methods HPLC method was used . The separation was performed on a Dinamonsil-C18 and column, methanol-water （550 ： 470） 1 000 mL. C7H15SO3 Na 960 mg and CH3COONa 82 mg were add HOAc 0.57 mL as the mobile phase; the flow rate was 1.0 mL · min^-1 ; the detection wavelength was 276 nm, and the column temperature was 28℃. Results Atenolol showed a good linearity in 0. 180 0-1. 800 0μg. Y= 0. 078 1 X （r=0. 999 9）; the average recovery rate was 97.08%, and the RSD was 1.33% （n=9）. Conclusion The method is simple, accurate, and it is suitable to determine the content of atenolol.
出处
《中南药学》
CAS
2007年第2期116-118,共3页
Central South Pharmacy
