摘要
目的观察小鼠肺组织中β防御素-4(mBD-4)和mBD-6的基因表达水平,以及急性肺损伤(ALI)对其表达的影响。方法将60只成年昆明小鼠随机分为ALI组和对照组(每组30只),用脂多糖腹腔注射复制ALI模型,于不同时间点处死小鼠获取肺组织,实时荧光定量PCR检测肺组织mBD-4、mBD-6的基因表达水平,测序鉴定PCR产物的特异性。结果对照组肺组织中各时间点及ALI组6 h点均无mBD-4、mBD-6基因表达,而ALI组12 h,1 d和3 d时间点表达显著升高(依次为mBD-4:0.032 6±0.010 4,0.048 7±0.012 6,0.046 8±0.009 2;mBD-6:0.039 7±0.008 3,0.044 4±0.007 2,0.042 5±0.011 3)。ALI组小鼠mBD-4表达在12 h时间点显著低于1 d和3 d时间点(P均<0.05),在1 d与3 d时无显著差异(P>0.05);ALI组mBD-6表达在12 h,1 d和3 d时间点无显著差异(P>0.05)。结论mBD-4和mBD-6基因在昆明小鼠肺组织中没有组成型表达,而ALI可以诱导其表达。
Objective To investigate the gene expression of beta-defensin-4 （mBD-4） and mBD-6 in mouse model of acute lung injury （ALI）. Methods Sixty adult mice were randomly divided into control group and ALI group. ALI was induced by intraperitoneal injection of lipopolysaccharide while the control group was treated with normal sahne. The lung tissues were harvested at different time point after stimulation. The expressions of mBD-4 and mBD-6 mRNA were measured by real-time quantitative RT-PCR. DNA sequencing was used to confirm the specificity of mBD-4 and mBD-6 cDNA fragment. Results There were no obvious mBD-4 and mBD-6 mRNA expression in mouse lung in the control group at all time points and ALI 6 h group.In the ALI group a marked increasing expression was found at 12 h, on 1 d and 3 d after LPS stimulation. The mBD-4 mRNA expression was significant higher on 1 d and 3 d than that at 12 h in the ALI groups. There were no significant differences of mBD-6 mRNA expression between ALI groups at 12 h, on 1 d and 3 d. Canclusion There is no constitutive expression of mBD-4 and mBD-6 mRNA in mouse lung, which could be induced in ALI.
出处
《中国呼吸与危重监护杂志》
CAS
2007年第2期131-134,I0002,共5页
Chinese Journal of Respiratory and Critical Care Medicine
关键词
急性肺损伤
Β防御素
聚合酶链反应
基因表达
小鼠
Acute lung injury
β-defensin
Polymerase chain reaction
Gene expression
Mouse
