摘要
目的:探讨ADAM28在牙源性上皮和间充质细胞中的表达差异,明确其亚细胞定位情况。方法:采用免疫细胞化学、免疫荧光和RT-PCR技术,从蛋白和基因水平检测ADAM28在人牙乳头细胞(HDPC)、牙囊细胞(HD-FC)、牙髓干细胞(HDPSC)、牙周膜细胞(HPDLC)和颈环上皮细胞(HDCLEC)的表达分布。结果:ADAM28在上述五种细胞的胞浆和胞膜上均有强弱不等的阳性表达。结论:ADAM28可能作为一种胞浆蛋白调控着牙源性细胞的增殖与分化,在牙胚发育中起重要作用。
Objective：To investigate the expression differences of ADAM28 in odontogenic epithelial and mesenchyreal cells and definitude the sub-cell orientation of ADAM28.Method： Immunocytochemistry, immunofluorescence and RTPCR were performed to detect the expressions of ADAM28 in HDPC, HDFC, HDPSC, HPDLC and HDCLEC at protein and gene levels.Result： ADAM28 was expressed positively at different levels in cytoplasms and membranes of HDPC, HDFC, HDPSC, HPDLC and HDCLEC.Condusion： ADAM28, as a plasmosin, might regulate proliferation and differentiation of odontogenic cells and play a crucial role in tooth germ development.
出处
《临床口腔医学杂志》
2007年第3期139-142,共4页
Journal of Clinical Stomatology
基金
国家自然科学基金资助项目(30572046)
