摘要
目的:探讨僵蚕抗凝成分(ACBB)对大鼠内毒素休克伴弥散性血管内凝血(DIC)的保护作用。方法:60只雄性SD大鼠被随机分为空白对照组、模型组及ACBB高、中、低剂量组,每组12只。采用静脉注射脂多糖(LPS)的方法复制大鼠内毒素休克伴DIC模型。ACBB高、中、低剂量组于注射LPS即刻分别由静脉注射ACBB 36、18和9 mg/kg;空白对照组给予等量生理盐水。注射LPS前及LPS后1、3和6 h记录大鼠平均动脉压(MAP);并于LPS后6 h经颈总动脉取血,测定血浆副凝实验(3P实验)阳性率、D-二聚体含量、血小板计数(PLT)、纤维蛋白原(Fbg)含量及抗凝血酶(AT-)活性。结果:与空白对照组比较,随时间延长,模型组MAP显著下降,6 h PLT、Fbg、AT-均显著降低,D-二聚体、3P实验阳性率均明显升高,各指标比较差异均有显著性(P均<0.01)。与模型组比较,ACBB各剂量组可明显抑制LPS引起的MAP降低,抑制3P实验阳性率,增加D-二聚体含量,防止休克后PLT、Fbg、AT-活性降低,两组比较差异均有显著性(P均<0.01),且ACBB高剂量组各指标几乎接近于空白对照组。结论:ACBB对内毒素休克伴DIC大鼠具有保护作用。
Objective： To investigate the protective effect of the anticoagulant components of bombyx batryticatus （僵蚕, ACBB） on endotoxic shock complicated with diffuse intravascular coagulation （DIC） in rats. Methods ： Sixty SD rats were randomly divided into blank control group, model group, high, middle and low doses of ACBB group （each group n= 12）. The animal model with endotoxic shock was reproduced by lipopolysaccharide （LPS） intravenous injection. Then, high, middle and low doses of ACBB （36, 18 and 9 mg/kg） were intravenously injected to observe its protective effects on the pathological changes due to LPS injection. The same amount of normal saline was given to blank control group. Mean arterial pressure （MAP） was recorded before and 1, 3 and 6 hours after LPS injection. Positive rate of plasma protamine paracoagulation test （3P test）, D-dimer, platelet count （PLT）, plasma fibrinogen （Fbg）content and antithrombin Ⅲ （AT - Ⅱ ） activity were detected in blood collected from the common carotid artery 6 hours after LPS injection. Results： Compared with blank control group, MAP levels were significantly decreased in model group at different time points, PLT, Fbg and AT -Ⅱ were lowered, while D -dimer and positive rate of 3P test were elevated in model group 6 hours after LPS injection, and the differences were significant （all P〈0. 01）. Compared with the model group, ACBB significantly inhibited the decrease of MAP induced by LPS. ACBB also remarkably attenuated the positive rate of 3P test, increased D -dimer content, and prevented the decrease of the PLT, Fbg, and activity of AT -Ⅲ after shock （all P〈0. 01）. The indexes mentioned above in the high - dose ACBB group were nearly close to those of blank control group. Conclusion： These facts demonstrate that ACBB can protect rats from endotoxic shock complicated with DIC.
出处
《中国中西医结合急救杂志》
CAS
2007年第2期80-82,共3页
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
基金
国家自然科学基金资助项目(30171159)
关键词
僵蚕抗凝成分
内毒素休克
保护作用
大鼠
anticoagulant components of bombyx batryticatus
endotoxic shock
protective effect
rats
