摘要
目的:探讨黄芪多糖拮抗绿脓杆菌在呼吸道黏附,阻断感染作用及机制,从而证明黄芪多糖是补益类中药黄芪中起以上作用的主要成分。方法:复制慢性支气管炎(慢支)感染绿脓杆菌小鼠模型。从120只模型小鼠中随机选择20只进行细菌定量培养,观察感染后35 d内小鼠气管带菌情况。另100只小鼠随机再均分为5组。低、中、高剂量治疗组分别以0.26、0.78和1.56 mg黄芪多糖灌胃,阳性对照组以慢支固本冲剂1 ml灌胃,阴性对照组以1 ml蒸馏水灌胃,各组均连用10 d。从各组随机取10只小鼠,先取其气管组织作细菌定量培养,观察小鼠气管带菌数;再取其气管标本进行病理切片,用光学显微镜观察气管黏膜上皮的病理变化。将各组另10只小鼠进行活体气管和支气管原位固定,用扫描电镜观察其超微结构及绿脓杆菌黏附数。结果:1各治疗组细菌培养菌落形成单位数明显低于阴性对照组(P均<0.01),高剂量治疗组与阳性对照组差异无显著性(P>0.05)。2光镜观察:各治疗组气管黏膜的病理变化轻于阴性对照组;高剂量治疗组和阳性对照组病变基本消失。3电镜观察:治疗组纤毛损伤、黏附的绿脓杆菌数明显少于阴性对照组,与阳性对照组相似。结论:黄芪多糖对慢支病变小鼠气管黏膜上皮有修复作用,能阻断绿脓杆菌在呼吸道的黏附,从而起到抗绿脓杆菌感染的作用;黄芪多糖是补益类中药黄芪中抗细菌黏附的有效成分。
Objective.. To explore the effects and mechanism of astragalus polysaccharides （APS,黄芪多糖） in prevention of pseudomonas （P.） aeruginosa infection in trachea by way of anti - bacterial adherence and to prove that APS is the major component acting above effects of astragalus in tonic medicine （补益类中药）. Methods： A chronic bronchitis model infected by P. aeruginosa was established. Twenty of 120 mice with P. aeruginosa infection were selected randomly to cultivate the bacteria quantitatively. Bacteria in trachea were observed within 35 days after infection. One hundred experimental animals were randomly divided into five groups （each n= 20）. Different concentrations of APS （0.26, 0.78 and 1.56 mg） were perfused into stomach in low, middle and high dose groups respectively. Manzhi Guben medicinal granules （慢支固本冲剂） 1 ml was given to the 4 th group as the positive control group. No medicine but 1 ml water was administered in the 5 th group as the negative control group. All groups took 10 days for the experiment. Then, the tracheal samples from 10 mice of each group were taken to cultivate the P. aeruginosa, to judge the bacterial number and to examine the microscopic pathological changes in mucosal epithelium of trachea. The other 10 mice tracheal samples of each group were taken to observe the tracheal ultrastructure and P. aeruginosa adherence by scanning electron microscope. Results： ①The numbers of clonal formation units of APS groups were much lower than that of negative control group （all P〈0. 01）, but there was no statistical difference between the high -dose APS group and positive control group （P〉0. 05）. ②Under optical microscope, the pathological changes of the trachea mucosa of treatment groups were milder than those of negative control group. Pathological changes were not seen in high -dose APS group and positive control group. ③Under scanning electron microscope, the injuries of cilia and the adhered number of P. aeruginosa in trache
出处
《中国中西医结合急救杂志》
CAS
2007年第2期76-79,F0002,共5页
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
基金
浙江省中医药管理局重点资助项目(052005)
关键词
黄芪多糖
绿脓杆菌
细菌黏附
动物实验
呼吸道感染
astragalus polysaccharides
pseudomonas aeruginosa
adherence of bacteria
animal experiment
trachea infection
