摘要
目的:建立以高效液相色谱法测定左旋多巴包合物中主药含量的方法。方法:色谱柱为C18,流动相为甲醇-0.01mol.L-1磷酸二氢钾缓冲液(25∶75,用磷酸调pH至3.0),流速为1.0mL.min-1,柱温为30℃,紫外检测波长为280nm,进样量为20μL。结果:左旋多巴的线性范围为5～80μg.mL-1(r=0.9998),平均回收率为100.1%(RSD=0.19%,n=15)。结论:本方法简便、快速、准确,可用于该包合物的质量控制。
OBJECTIVE： To establish a HPLC method for the determination of levodopa in levodopa inclusion complex. METHODS： The separation was performed on C18, and the mobile phase consisted of methanol- 0.01mol · L^-1 potassium dihydrogen phosphate buffer solution （25 ： 75, pH 3.0） with a flow rate of 1.0mL· min^-1. The column temperature was 30℃, the detection wavelength was 280 nm and the sample size was 20μL. RESULTS： A good linear relationship was obtained for levodopa within the range of 5-80μg · mL^-1（r = 0.999 8）, and its average recovery was 100.1% （RSD = 0.19%, n = 15）. CONCLUSION： This method is simple, rapid, accurate, and it can be used for the content determination of levodopa in levodopa inclusion complex.
出处
《中国药房》
CAS
CSCD
北大核心
2007年第10期770-771,共2页
China Pharmacy
基金
山东省优秀中青年科学家科学奖励基金项目(03BS050)
