摘要
目的:探讨PKC-α与P-gp在紫杉醇诱导的卵巢癌耐药细胞系A2780/Taxol中的表达.方法:免疫细胞化学SP法及免疫荧光双标检测PKC-α与P-gp在耐药细胞A2780/Taxol及其亲本细胞中的表达;WesternBlot检测并半定量分析PKC激动剂佛波脂(PMA)和抑制剂十字孢碱(SP)作用后P-gp在两种细胞上的表达水平.结果:免疫细胞化学结果表明在A2780/Taxol中,P-gp与PKC表达均呈阳性,而在亲本细胞中,P-gp不表达,PKC呈阳性表达.在A2780/Taxol中PKC-α与P-gp有共表达.SP及PMA作用后,A2780/Taxol细胞P-gp表达无明显差别.结论:卵巢癌对紫杉醇耐药的产生与PKC-α和P-gp有关.
AIM： To detect the expressions of P-glycoprotein （P-gp） and protein kinase C-α （PKC- α ） isoform in Taxolresistant ovarian cancer cell line A2780/Taxol. METHODS： SP method and double-labeled immunofluorescence were used to determine the expressions of P-gp and PKC-α in A2780/Taxol and A2780. Western Blot was employed for assess P-gp expression after A2780/Taxol was co-cultured with phorbol myristate acetate （ PMA ） or staurosporine （ SP ）. RESULTS： There was a co-expression of P-gp and PKC-α in A2780/Taxol, but P-gp was not expressed in A2780. And the expression level of P-gp in A2780/Taxol was not affected by the presence of PMA and SP. CONCLUSION： P-gp and PKC-α play important roles in the resistance of ovarian cancer cell A2780 to taxol.
出处
《第四军医大学学报》
北大核心
2007年第6期527-529,共3页
Journal of the Fourth Military Medical University
关键词
卵巢癌
紫杉醇耐药
P-糖蛋白
蛋白激酶C
ovarian neoplasms
Taxol resistance, P-glycoprotein
protein kinase C
