摘要
目的:探讨外源性白细胞介素-10(IL-10)对大鼠重症急性胰腺炎(SAP)肠屏障功能的影响。方法:90只SD大鼠随机分为假手术组、SAP组I、L-10治疗组,制模12 h后,检测各组血生化指标、血浆内毒素水平、脏器细菌易位率、小肠黏膜细胞间黏附分子-1(ICAM-1)及观察各组胰腺、小肠病理形态学改变。结果:制模12 h后,SAP组血浆内毒素为(144.682±9.584)EU/L、脏器组织细菌易位率为64.6%、小肠黏膜ICAM-1 5.842±0.706、胰腺病理评分10.62±0.60、小肠病损严重。IL-10治疗组上述指标测定值分别为:(103.112±8.124)EU/L(P<0.05),30.6%(P<0.01),4.102±0.668(P<0.05),4.46±0.52(P<0.01)、小肠病损明显减轻。结论:小肠黏膜黏附分子异常表达及炎性介质平衡紊乱,参与SAP鼠肠屏障功能受损。早期应用外源性IL-10能降低小肠黏膜黏附分子表达,抑制炎性介质过度释放,减少肠源性细菌易位,对SAP鼠肠屏障功能有保护作用。
Objective： To study the effect of exogenous IL-10 on gut barrier in severe acute pancreatitis （SAP）. Methods： Ninety SD rats were randomly divided into three groups as the sham-operation group, SAP group and SAP with IL-10 treated group. Tweleve hours after operation, the indices in every group were recorded as following： the biochemical index, plasma endotoxin, organs bacterial translocation rate, ICAM-1 expression in gut mucosa, and the pathologic changes in pancreas and gut. Results： In SAP group, the plasma endotoxin was （144. 682±9. 584）EU/L, bacterial translocation rate was 64.6%, ICAM-1 expression （by flow cytometry） was 5. 8424-0.706, pancreas pathology score was 10. 62±0. 60, gut damage under microscope was severe. In IL-10 treated group, the above indices were significantly decreased as 103. 112±8. 124（P〈0.05）, 30.6% （P〈0.01）, 4. 102±0. 668（P〈0.05） and 4.46±0.52（P〈0.01）, respectively, and the＇gut damage was ameliorated. Conclusion： The abnormal expression of ICAM in gut mucosa and the disorder of inflammatory interleukin both participated in the gut barrier disfunction during SAP. Early thearpy with exogenous IL-10 could alleviate ICAM-1 expression in gut mucosa, prevent inflammatory interleukin＇s releasing and decrease the bacterial translocation, which suggested a protective effect of IL-10 on gut barrier in SAP rats.
出处
《武汉大学学报（医学版）》
CAS
2007年第2期159-161,共3页
Medical Journal of Wuhan University
