摘要
目的：建立黄芪注射液浓缩液微乳中黄芪甲苷的含量测定方法。方法：采用（HPLC-ELSD）。色谱柱：Zorbax C18柱（4．6min×250mm，5μm）；流动相：乙腈：水（36：70）；检测波长：206nm；灵敏度为O．08AUFS，流速：1．0ml／min；柱温：25℃。ELSD参数：漂移管温度为55℃，空气气压280kPa。结果：黄芪甲苷在0．0041456-0．0404560mg／ml时有良好的线性关系（r=0．9991），平均回收率为96．53％，RSD为0．76％。结论：HPLC—ELSD法具有准确、简便、灵敏度高、无干扰而且重现性好的优点，适合用于黄芪注射液微乳中黄芪甲苷含量的测定。
Objective：To establish a method for determining astragaloside IV in injection astragalus microemulsion. Methods ： HPLC-ELSD was employed in the quantitative analysis of astragaloside IV in the sample. The separation was performed on Zorbax C 18 （4.6 mm× 250 ram, 5 μm） column with a mixture of acetonitrile-water（ 36： 70） as mobile phase. Wavelength：206 nm; response 0.08 AUFS. The flow rate was 1.0 ml/min . ELSD parameter： The temperature of drift tube for measurement was 55℃, air pressure： 280 kPa, CAIN6. Results： The standard curve was linear in the range of 0. 004 145 6 - 0. 040 456 0 mg/ml, r = 0. 999 1. The average recovery was 96.53%, RSD： 0.76%. Conclusion： The method is simple, reliable, sensitive, free from interference and it have good reproductivity. Therefore it is fit to determine astragaloside IV in microemulsion.
出处
《安徽中医学院学报》
CAS
2007年第2期36-38,共3页
Journal of Anhui Traditional Chinese Medical College
基金
国家自然科学基金项目(C03050203)
