摘要
目的探讨系统性红斑狼疮(SLE)外周血中Th1/Th2/Th3型细胞因子网络平衡及其与疾病的关系。方法采用酶联免疫吸附试验(ELISA)测定54例SLE患者及20例正常对照外周血清白细胞介素-4(IL-4)、干扰素-γ(IFN-γ)及转化生长因子-β(TGF-β)表达水平。结果(1)SLE患者血清IL-4、IFN-γ的表达水平高于健康对照组(P<0.01),血清TGF-β的表达水平显著低于健康对照组(P<0.01),IL-4/IFN-γ的比值高于健康对照组(P<0.01);(2)SLE血液系统受累组TGF-β的表达水平低于血液系统未受累组(P<0.05);抗ds-DNA阳性组IFN-γ的表达水平高于抗ds-DNA阴性组(P<0.05),IL-4/IFN-γ比值及TGF-β的表达水平低于抗ds-DNA阴性组(P<0.05)。结论SLE患者的IL-4、IFN-γ表达水平增高,TGF-β的表达水平降低,存在明显的细胞因子网络平衡失调,Th1/Th2/Th3细胞因子网络出现异常在SLE的发病过程中具有一定意义。
Objective To investigate the polarization of Th1/Th2/Th3 cell function in the peripheral blood of systemic lupus erythematosus. Methods The levels of IL-4 ,IFN-γand TGF-βin the peripheral blood were detected by Sandwich ELISA in 54 SLE and 20 healthy subjects. Results①The levels of IL-4 and IFN-γin the peripheral blood from the patients of SLE （ n = 54 ） were significantly increased, compared with those from normal controls （ n =20） （ P 〈0.01 ）. The level of TGF-β was significantly lower than that from normal controls（ P 〈0.01 ）. The ratio of IL-4 to IFN-γwas significantly higher than that from normal controls （ P 〈 0.01 ）. ②The level of TGF-β in the pe ripheral blood from the patients of SLE with hematological disorders was lower than those without hematological disorders. The level of IFN-γin anti-dsDNA（ ＋ ） group was higher than that in anti-dsDNA（ - ） group. The ratio oflL- 4 to IFN-γand the level of TGF-β in anti-dsDNA （ ＋ ） group were lower than those in anti-dsDNA （ - ） group. Conclusion There is imbalance of Th1/Th2/Th3 cytokines in the patients with SLE：levels of IL-4 and IFN-γare higher with lower TGF-β than those in normal controls.
出处
《中国临床保健杂志》
CAS
2007年第2期149-151,共3页
Chinese Journal of Clinical Healthcare
基金
安徽省临床医学重点学科应用技术项目(05A008)
关键词
红斑狼疮
系统性
白细胞介素4
干扰素Ⅱ型
转化生长因子Β
Lupus erythematosus ,systemic
Interleukin-4
Interferon type Ⅱ
Transforming growth factor beta
