摘要
目的:研究赤芍总苷(TPG)提取液体外清除自由基和抗凝血的活性。方法:通过相同溶剂不同方法提取TPG,采用二苯基三硝基苯肼(DPPH)自由基体系、超氧阴离子自由基体系、羟基自由基体系检测TPG清除自由基的活性,同时测定TPG提取液对凝血酶原时间(PT)、部分凝血酶时间(APTT)、凝血酶时间(TT)的影响。结果:沸水浴0.5h提取2次,TPG含量为4.682%;采用不同方法得到的TPG提取液对DPPH清除率为92.36%～96.65%,抗超氧阴离子活力和活性氧活力分别为415.15～496.97U.L-1和774.32～1911.60U.L-1;当TPG浓度≥0.012g.L-1时可显著延长PT(P<0.05),当TPG浓度≥0.0012g.L-1时可显著延长APTT(P<0.05)和TT(P<0.01)。结论:采用不同方法得到的TPG提取液均有较强的清除自由基和抗凝血活性。
OBJECTIVE ： To analyze and compare the free radical - scavenging and anticoagulant activities of Total paeony glycoside （TPG） extracts in vitro. METHODS： TPG was extracted with the same solvent but different extracting methods, and free radical- scavenging and anticoagulant effects of different extracts were detected by the assay systems of DPPH, superoxide radical and hydroxyl radical as well as PT, APTT and TT test. RESULTS： The extraction rate of TPG boiling in water twice was 4. 682%. The effects on scavenging free radical were 92.36%- 96.65% （DPPH radical）, 415.15-496.97 U·L^-1（superoxide radical ） and 774.32-1 911.60 U·L^-1（hydroxyl radical） respectively, and the TPG extracts also prolonged PT （TPG≥0.012g·L^-1,P〈0.05）,APTT （TPG≥0.001 2g · L^-1,P〈0.05） and TT（TPG≥0.001 2g ·L^-1,P〈0.01）. CONCLUSION： TPG extracts have obvious free radical- scavenging and anticoagulant activities, but different extracting methods may influence the free radical - scavenging and anticoagulant activities and mechanism.
出处
《中国药房》
CAS
CSCD
北大核心
2007年第9期643-646,共4页
China Pharmacy
基金
陕西省中医管理局基金项目(2005007)
西安市工业科技计划项目(GG04066)
关键词
赤芍总苷
自由基
抗凝血
提取方法
Total paeony glycoside （TPG）
Free radical
Anticoagulant
Extracting methods
