摘要
目的 研究阻塞性睡眠呼吸暂停低通气综合征（OSAHS）患者红细胞天然免疫黏附活性（erythrocyte innate immune adhesion function，EIIAF）的变化及和红细胞补体Ⅰ型受体（erythrocyte complement receptor type 1，C3bR，CD35，ECR1）数量变化之间的关系，探讨引起红细胞天然免疫功能变化的可能原因。方法 采用郭峰法和流式细胞仪间接免疫荧光法测定33例OSAHS患者和30例正常对照者外周血红细胞CR1活性及其数量。结果 OSAHS患者红细胞C3b受体花环率（RBC-C3bRR）（9．6±2．1）％明显低于正常对照者（11．2±2．2）％（P值〈0．01），红细胞免疫复合物花环率（RBC-ICR）（13．9±4．2）％明显高于正常对照者（9．7±2．1）％（P值〈0．01），红细胞CD35数量（ECD35）（41．4±7．6）％低于正常对照者（47．7±8．1）％（P值〈0．01），且OSAHS患者RBC-ICR水平与平均血氧饱和度（MSaO2）、最低血氧饱和度（LSaO2）、血氧饱和度〈90％时间占总睡眠时间百分比（SIT90）均有显著相关性（r=-0．438、-0．485、0．494，P值〉〈0．05、0．01、0．01），ECD35水平与MSaO2、LSaO2、SIT90有显著相关性（r=0．383、0．542、-0．395，P值〈0．05、0．01、0．05）。结论 OSAHS患者红细胞天然免疫功能下降，原因可能与夜间反复缺氧有关。
Objective To investigate the erythrocyte innate immune function in the patients with obstructive sleep apnea-hypopnea syndrome（OSAHS）. Methods Complement receptor type 1 on erythrocyte （ECR1） in the fresh peripheral blood was detected in 33 OSAHS patients and 30 normal controls by Guo-Feng methods and FCM technique respectively. The immune function of red blood cells was detected, including the rossett rate of red-cell C3b receptors（RBC-C3bRR）, red-cell immune complex（RBC-ICR） and the expression level of erythrocyte CD35. Results Compared with those of control group,the levels of RBC- C3bRR and ECD35 in OSAHS patients were significantly lower,respectively （9.6±2.1）M vs （11.2±2.2）%（ P 〈0.01）, （41.4±7. 6） % vs （47. 7±8. 1） % （ P〈0.01） ,and the level of RBC-ICR was significantly higher （ P 〈 0.01）. In the patients with OSAHS, RBC-ICR level correlated significantly with MSaO2, LSaO2, SIT90（ r = -0.438, -0. 485,0. 494, P 〈0.05,0.01,0.01）, ECD35 level correlated significantly with MSaO2, LSaO2, SIT90 （ r=0.383,0.542,-0.395, P 〈0.05,0.01,0.05）. Conclusions OSAHS patients show impairment of the erythrocyte innate immune function,which may be caused by hypoxia.
出处
《国际呼吸杂志》
2007年第5期324-326,共3页
International Journal of Respiration
