摘要
用高效液相色谱法建立了氯硝安定血浓度测定法。本法以茶碱为内标，用二氯甲烷提取，氮气吹干，流动相溶解进行测定。色谱条件：国产ＹＷＧ—ＳｉＯ２柱（４．６ｍｍ×２５０ｍｍ），紫外检测波长２５４ｎｍ，灵敏度０．０２０ＡＵＦＳ，流动相为正己烷—无水乙醇（５２∶４８）。血药浓度在１０．０～２００．０μｇ／Ｌ范围内线性良好，日内相对标准偏差为２．０％～４．９％，日间相对标准偏差为２．３％～５．２％，最低检测限１．０μｇ／Ｌ。本法灵敏度较高，用血量少，操作简便，已应用于临床治疗药物监测。
A simple, sensitive and rapid normal phase HPLC method for the determination of clonazepam (CZP) in human serum is described, using YWG-SiO2 column (4. 6mm×250mm) and UV detector at 254nm. The mobile phase was hexane-ethanol (52 : 48), the flow rate was 0. 9ml/min. The linear range of drug concentration in serum was 10. 0～200.0μg/L (r=0. 9993) and detection limit was 1.0μg/L. The recovery rate of CZP was 99. 2%～101. 3%, the RSD of within-day and between-days were 2. 0%～4. 9% and 2. 3% ～5. 2%, respectively. The method was already used in clinical practices.
出处
《药物分析杂志》
CAS
CSCD
北大核心
1996年第4期244-246,共3页
Chinese Journal of Pharmaceutical Analysis
