摘要
目的:通过探讨血卟啉衍生物光动力学效应对人鼻咽癌细胞株CNE2的生物作用,为光动力治疗放、化疗效果较差的鼻咽癌提供理论依据。方法:实验于2005-10/2006-06在南方医科大学南方医院肿瘤生物治疗中心实验室完成。对体外培养的人鼻咽癌细胞株CNE2分为光处理和无光处理各8个实验组及各分别设对照组和空白对照组,实验组分别加入0.5,1.0,1.5,2.0,2.5,3.0,3.5,4.0mg/L血卟啉衍生物100μL,每组设7个复孔;孵育4h,对照组除不加光敏剂外其他与实验组相同,空白对照组只加培养基不加细胞和光敏剂。光处理实验组给予波长为630nm的半导体激光进行照射,照射的功率密度为20mW/cm2,照射能量密度分别为20,10,5,2J/cm2,继续孵育24h,用四唑盐(MTT)比色法测定细胞存活率。细胞存活率(%)=(实验组A值-空白对照组A值)/(对照组A值-空白对照组A值)×100%。每次实验重复3次,取平均值。结果:①血卟啉衍生物与CNE2细胞共同孵育后,采用630nm的半导体激光照射可对其产生杀伤作用,杀伤作用大小与血卟啉衍生物的浓度和激光剂量成正相关(P<0.01),但血卟啉衍生物浓度达2.5mg/L以上时杀伤细胞作用无显著增加。激光能量为20J/cm2,血卟啉衍生物的浓度为2.5mg/L时,其杀伤CNE2细胞的作用最明显。②在加入血卟啉衍生物而无激发光的情况下,血卟啉衍生物对鼻咽癌细胞生存不产生明显影响(P>0.05)。结论:血卟啉衍生物介导的光动力在体外可以对CNE2细胞产生杀伤效应,在一定范围内杀伤作用大小与血卟啉衍生物的浓度和激光剂量成正相关。
AIM： TO explore the physical effect of hematoporphyrin derivative photodynamic therapy on CNE2 cell line of human nasopharyngeal carcinoma, so as to provide theoretic evidence for the photodynamic treatment of nasopharyngeal carcinoma having a bad curative effect with chemotherapy.
METHODS： The experiment was conducted in the laboratory of Tumor Biotherapy Center, Nanfang Hospital, Southem Medical University between October 2005 and June 2006. CNE2 cells were divided into 8 experiment groups; meanwhile, the control group and bland control group were established, respectively. The cells of the experiment groups were incubated in a medium contained of 100μL various concentrations of hematoporphyrin derivative （0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5, 4.0 mg/L） with 7 holes in each group. Four hours later, the control group was given the previous treatment except photosensitive drug, and the blank control group was only given medium without cells and photosensitive drug, while the experiment groups were illuminated with different light doses （20, 10, 5, 2 J/cm^2） with a semiconductor laser at 630 nm with the energy density of 20 mW/cm^2. After 24 hours incubation with hematoporphyrin derivative photodynamic therapy, the survival rates of CNE2 cells were analyzed by MTT assay： survival rates （%）= （A value of experiment groups-A value of blank control group）/（A value of control group-A value of blank control group）×100%. The experiment was repeated for three times to take the average values.
RESULTS： （1）After co-culture of hematoporphyrin derivative and CNE2 cells, irradiation with isemiconductor laser at 630 nm could kill the CNE2 cells. The killing effect was positively correlated with the concentration of hematoporphyrin derivative and the dosage of radiation （P 〈 0.01）, but it did not increased obviously when the concentration of hematoporphydn derivative was over 2.5 mg/L. It displayed the strongest killing effect under the condition of 20 J/cm^2 radiation and 2.5 mg/L hematop
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2007年第9期1707-1709,共3页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
中国临床肿瘤学科学基金(Y-2005-0013)~~
