摘要
采用反复冻融和超声波破碎法破碎17种微藻细胞,通过细胞破碎率和抗菌活性检测破碎效果,以选择适合不同微藻的破碎方法,利于胞内活性物质的提取．细胞破碎结果显示:经过12 min的超声处理,所有实验藻种的破碎率均在90%以上,特别是扁藻、角毛藻以及球等鞭金藻和甲藻,仅处理3min,破碎率达到了99%以上,更适合超声波法破碎．反复冻融法破碎藻细胞,经过2次冻融,10种微藻的破碎率在95%以上,4种在60%～80%,3种小于40%,对此三种藻(分别是扁藻,小球藻和紫球藻)的破碎应选择超声或组合法;以金黄色葡萄球菌(S．aureus)为指示菌,采用琼脂扩散法检测细胞破碎率对抑菌活性的影响,抗菌活性表明:细胞充分破碎,有利于活性物质的释放和提取．
For efficient recovery active substance from alga,seventeen species of alga were crushed by ultrasonication and freezing and thawing,and their crushing effects was determined by the crushing efficiency and antimicrobial activity,to choose appropriate method for different alga. The results of crushing alga cells showed that fragmentation efficiency of all of tested alga cells was more than 90% after treatment 12 minute using ultrasonic method,moreover,fragmentation efficiency was more than 99% only treated 3 minute for the alga,such as Platymonas subcordiformis, Chaetoeoreros, Isoehrysis galbana and Zooxanthella rnicroadn＇atica, so the crushing method was more appropriate to these alga compared with the freezing and thawing method. Using freezing and thawing method duty cycle twice,fragmentation efficiency of ten-species alga increased over 95%, four species was between 60%-80%,three species was less than 40%,indicating that crushing method of the three alga（Platymonas subeordiformis, Chlorella saccharophila, Porphyridium cruentum）should choose other or combination methods. And the results of antimicrobial activity,which determined against S. aureus using agar diffusion method,indicated that the sufficient crushing efficiency of alga cells was propitious to releasing and distilling of active substances.
出处
《天津科技大学学报》
CAS
2007年第1期21-25,共5页
Journal of Tianjin University of Science & Technology
基金
天津市科委应用基础重点资助项目(043804211)
天津市科技攻关项目(06YFGZNC04200)
关键词
超声破碎
反复冻融
微藻
活性物质
ultrasonication
freezing and thawing
microalgae
bioactive substance
