摘要
An acidophilic,rod-shaped Gram-negative sulfur oxidizing strain BY-05 was isolated from an acid mine drainage of copper ore in Baiyin area,Gansu Province,China.Ultrastructural studies show that the isolate has a tuft of polar flagella and possesses sulfur granules with clear membrane adhering to the cell innermembrane.Physiological study shows that this isolate grows autotrophically and aerobically by oxidizing S0and reduced inorganic sulfur compounds(SO, 2 23-SO, 2 24- S2 -and ZnS)with the optimum growth at pH 3.5-4.0 and at the temperature range of 25-30℃.The 16S rRNA gene sequence(DQ 423683)of strain BY-05 has 100%sequence similarity to that of Acidithiobacillus albertensis(DSM 14366).So it is identified and named as A. albertensis BY-05.Bioleaching experiments with this new strain show that it can play an important role in recovery of metals from chalcopyrite and sphalerite.
An acidophilic, rod-shaped Gram-negative sulfur oxidizing strain BY-05 was isolated from an acid mine drainage of copper ore in Baiyin area, Gansu Province, China. Ultrastructural studies show that the isolate has a tuft of polar flagella and possesses sulfur granules with clear membrane adhering to the cell irmermembrane. Physiological study shows that this isolate grows autotrophically and aerobically by oxidizing So and reduced inorganic sulfur compounds （S2O3^2-, S2O4^2-, S^2- and ZnS） with the optimum growth at pH 3.5-4.0 and at the temperature range of 25-30 ℃. The 16S rRNA gene sequence （DQ 423683） of strain BY-05 has 100% sequence similarity to that of Acidithiobacillus albertensis （DSM 14366）. So it is identified and named as A. albertensis BY-05. Bioleaching experiments with this new strain show that it can play an important role in recovery of metals from chalcopyrite and sphalerite.
出处
《中国有色金属学会会刊：英文版》
EI
CSCD
2007年第1期168-175,共8页
Transactions of Nonferrous Metals Society of China
基金
Project(50321402)supported by the National Natural Science Foundation of China
Project(2004CB619204)supported by the NationalBasic Research Program of China
关键词
硫化物
矿石
生物浸取
金属
选矿工艺
黄铁矿
sulfur oxidation
Acidithiobacillus albertensis
Acidithiobacillus thiooxidans
isolation and characterization of bacteria
bioleaching
