摘要
建立HPLC法测定复方茶碱麻黄碱片中可可碱、盐酸麻黄碱、茶碱和咖啡因的含量。采用Diamonsil C18色谱柱，以0．05mol·L^-1磷酸二氢钾-甲醇-三乙胺（77：23：0．2）为流动相，检测波长为215nm。线性范围为可可碱：0．1011—0．9097μg（r=0．9998）；盐酸麻黄碱：0．0418—0．3758μg（r=0．9999）；茶碱：0．0973—0．8759μg（r=0．9999）；咖啡因：0．0614—0．5522μg（r=0．9999），平均回收率为可可碱：99．8％（RSD=0．6％，n=9）；盐酸麻黄碱：100．4％（RSD=0．9％，n=9）；茶碱：99．6％（RSD=0．5％，n=9）；咖啡因100．1％（RSD=0．3％，n=9）。本方法简便、快速，专属性强，可有效地控制复方茶碱麻黄碱片中可可碱、茶碱、咖啡因和麻黄碱的含量。
A HPLC method was established for determination of theobromine, ephedrine hydrochloride, theophylline and caffeine in Compound Theophylline and Ephedrine Hydrochloride Tablets. A Diamonsil C18 column was used with 0. 05mol · L^-1 potassium dihydrogen phosphate, methanol and triethylamine （77： 23： 0. 2） as the mobile phase. The wavelength of detection was at 215nm. A good calibration linearity and correlation coefficient were obtained which theobromine was at 0. 1011-0. 9097μg （0. 9998 ）, ephedrine hydrochloride at 0. 0418 - 0. 3758μg （ 0. 9999 ）, theophylline at 0. 0973 - 0. 8759μg （ 0. 9999 ） and caffeine at 0. 0614 - 0. 5522μg （0. 9999 ）. The recoveries were 99. 8% （ RSD% = 0. 6% ） for theobromine, 100. 4% （ RSD% = 0. 9% ） for ephedrine hydrochloride, 99. 6% （RSD% =0. 5% ） for theophylline and 100. 1% （RSD% =0. 3% ） for caffeine. The method is convenient and accurate, and could be used on the quality control.
出处
《中国药事》
CAS
2007年第2期101-102,111,共3页
Chinese Pharmaceutical Affairs
关键词
高效液相色谱法
复方茶碱麻黄碱片
可可碱
盐酸麻黄碱
茶碱
咖啡因
含量测定
Compound Theophylline and Ephedrine Hydrochloride Tablets
theobromine
ephedrine hydrochloride
theophylline
caffeine
determination
HPLC
