摘要
以黄瓜种子为材料,采用甲硫氨酸(Methionine,Met)浸种的方法,研究了不同浓度Met对黄瓜种子萌发、幼苗生长及离体子叶成花的影响.结果发现:(1)10-4M Met处理3 h显著促进苗的生长而10-5M Met浸种处理则有利于子叶的生长;(2)10-5M Met浸种处理显著促进离体子叶成花;(3)离体子叶培养物的花芽分化力与子叶离体前的子叶/苗的鲜重比呈显著的正相关性(P<0.01);(4)添加10-5M DNA去甲基化试剂5-氮胞苷(5-Aza-cgtidine,5-Aza)能逆转10-5M Met处理对离体子叶培养物成花的促进作用.上述结果说明:10-5M Met浸种3 h能显著促进子叶培养物的花芽(无论直接花还是间接花)形成,Met对离体子叶成花的诱导作用可能是作为甲基供体通过DNA甲基化途径来实现的.
The effects of imbibition with different concentrations of methionine （Met） for 3 hours on seed germination, growth of cucumber seedlings, and flowering of detached cucumber cotyledon in vitro were studied. The following results were obtained： （1）As compared with the control, seedling growth was obviously improved by imbibition with 10^-4 M Met for 3 hours and cotyledon growth was improved by imbibition with 10^-5 M Met; （2）The formation of flower buds from detached cucumber cotyledons（either direct flowers or indirect flowers） was enhanced clearly by the soaking with 10^-5 M Met; （3） The positive correlation was found between ratio of fresh weight of cotyledon to seedling and the differentiation of flower buds from detached cotyledon culture（P〈0.01）; （4）Promotion of flowering of detached cucumber cotyledons by treatment with Met was fully removed by adding 10^-5 M 5- Azacgtidine, a DNA demethylating agent. This finding suggests that Met induce the flowering of detached cotyledon culture perhaps through acting as a methyl-donor of DNA methylating not as the precursor of ethylene synthesis.
出处
《浙江大学学报（理学版）》
CAS
CSCD
北大核心
2007年第2期219-222,227,共5页
Journal of Zhejiang University（Science Edition）
基金
浙江省自然科学基金资助项目(398082)
关键词
黄瓜子叶
甲硫氨酸
浸种
DNA甲基化
子叶培养物成花
cucumber cotyledon
methionine
imbibition
DNA methylating
flowering of cultures of detached cucumber
