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禽霍乱蜂胶灭活疫苗研制中的铁螯合剂适宜浓度确定 被引量:2

Development of a Novel Broad-spectrum Propolis Adjuvant Inactivated Vaccine against Fowl Cholera
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摘要 以含4%鸡血清的营养琼脂为培养基,进行了铁螯合剂2,2’-联吡啶对禽多杀性巴氏杆菌C48-3株的抑菌试验。结果表明,对于含4%鸡血清的营养琼脂培养基,铁螯合剂2,2’-联吡啶的合适添加量为200μmol/L。 Nutritious agar supplemented with 4% chicken serum was used as culture media. Suppressive effect of iron-chelator 2,2'-dipyridyl on pasteurella multocida strain C48 -3 was studied in order to find an easy method to confirm the appropriate concentration of iron -chelator supplemented in a specific culture media to induce expression of cross-protective factor (CPF) by pasteurella multocida. The result indicated that with this method, an appropriate concentration of iron-chelator could be found out for specific culture media to induce expression of CPF. As for nutritious agar culture media supplemented with 4% chicken serum, the appropriate concentration of 2,2'-dipyridyl was 200 μmol/L.
出处 《安徽农业科学》 CAS 北大核心 2007年第5期1378-1379,共2页 Journal of Anhui Agricultural Sciences
基金 安徽省教育厅自然科学研究项目"新型广谱禽霍乱蜂胶灭活疫苗的研制(2006KJ416B)"
关键词 螯合剂 2 2’-联吡啶 多杀性巴氏杆菌 Iron Chelator 2,2'-dipyridyl Pasteurellamultocida
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参考文献9

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二级参考文献2

  • 1吴范庚,钱心元.我国多杀性巴氏杆菌耐热抗原血清型(第一报)[J]中国兽医杂志,1987(12). 被引量:1
  • 2王秀贤,姚龙涛,唐水明,顾应泉.禽霍乱弱毒菌苗“731”株和禽霍乱弱毒菌苗“Cu”系免疫效果测定[J]中国兽医杂志,1985(10). 被引量:1

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