摘要
背景与目的:机体免疫细胞可识别并排斥肿瘤细胞,在临床微波凝固治疗肝癌后免疫细胞的局部浸润的变化和细胞免疫对临床疗效的影响值得探讨。本研究分析超声引导下微波凝固治疗肝癌局部免疫细胞浸润变化,并探讨了局部细胞免疫对临床疗效的影响。方法:超声引导微波凝固治疗原发性肝癌患者89例,于微波治疗前后进行肝穿组织活检,经免疫组化染色后比较治疗前后局部CD4+、CD57+和CD68+浸润变化,并观察肝癌复发情况,分析局部免疫细胞浸润程度在复发和未复发组间是否存在差异。结果:微波治疗后CD4+、CD57+和CD68+局部浸润程度较治疗前显著增加(P<0.05);微波术前,CD4+和CD57+在未复发组阳性细胞密度显著高于复发组(P<0.05)。结论:微波凝固治疗肝癌后局部免疫细胞浸润增加,且局部免疫细胞的浸润程度对其临床疗效可有一定的影响。
Background and purpose： Tumour cell is recognized and rejected by immunocytes. The local infiltration of immunocytes and the clinical curative effect after the percutaneous microwave coagulation therapy （PMCT） is worthy of further study. We investigated the local immune response after PMCT under ultrasound guidance and evaluated if the local immune response had any influence on the prognosis of patients. Methods： 89 cases of patients with hepatocellular carcinoma were treated by PMCT. At the time of the fourth week after PMCT and before PMCT, the patients were given percutaneous biopsy under ultrasound guidance. And the samples were stained by immunohistochemical methods. Then the infiltration of CD4^＋ ,CD57^＋ and CD68^＋ -of local treatment areas were compared before and after the treatment of PMCT. Meanwhile, the patients underwent follow-up to survey the possibility of recrudescence. Moreover, the authors analyzed the relation between the infiltration of immunocytes and recrudescence. Results： A few CD4 ,CD57 and CD68 positive cells were observed in the cancer stoma and tissues surroundings the liver before PMCT. After PMCT, the immunocytes of CD4 ,CD57 and CD68 positive ceils were significantly increased both in the cancer and the adjacent liver tissue. These immunocytes were enlarged in size. The infiltration of CD4^＋ ,CD57 ^＋ and CD68^＋ increased significantly in post-PMCT as compared with those of pre-PMCT（ P 〈 0.05）. Before PMCT, the positive expression of CD4^＋ and CD57^＋ in the non-recrudescent group were higher than those of the recrudescent group （P 〈 0.05）. Conclusions： The local cellular immunity can be further improved after the tumor has treated by PMCT and it would be related to the prognosis of the patients.
出处
《中国癌症杂志》
CAS
CSCD
2007年第2期135-138,共4页
China Oncology
关键词
肝癌
超声
微波
治疗
免疫
hepatocellular carcinoma
ultrasound
microwave
therapy
immunity
