摘要
目的通过动物实验研究探索肝脏肿瘤微波治疗后的残留肿瘤的生物学特性的变化规律,借此评价非完全性微波治疗的效果,并为治疗后的复查和再次治疗时机提供科学依据。方法建立小鼠微波治疗后肝脏残余肿瘤模型,并分时段检测肿瘤细胞的增殖细胞核抗原和基质金属蛋白酶,并将治疗前后的结果进行比较,观察其生物学特性的变化及与对照组小鼠的生存时间及肺部转移情况。结果PCNA、MMP-9在微波治疗后两项表达均呈一过性的降低。微波治疗组小鼠与对照组相比生存期平均延长57%,肺部转移率低于对照组。结论微波治疗后残留肿瘤细胞的增殖活性和侵袭能力在短期内有明显的降低,残留肿瘤生长和转移受到一定程度的抑制,肿瘤分化程度较治疗前有一定的改善,微波治疗即使不能一次灭活肿瘤,但在一定程度上可延长机体的生存时间。
Objective To investigate the changes in biological features of remnant tumor cells of H22 liver cancer after treatment with microwave ablation and evaluate the effects of the treatment on body to provide scientific basis for examination and re-treatment after the treatment. Methods Microwave ablation was performed on part of the tumor in mice and proliferating cell nuclear antigen （PCNA） and matrix metalloproteinase 9 （MMP 9） were determined with immunohistochemistry at different stages. The results were compared to observe the changes in biological features, survival time and rate of pulmonary metastasis in the microwave ablation group and the control group. Results Therates of positive expression of PCNA and MMP 9 were significantly lower in the microwave ablation group than in the control group. Meanwhile, they slightly increased in the microwave ablation group as compared with the control 15 d after the treatment. The survival time in the microwave ablationgroup was prolonged by 57% and associated with lower rate of pulmonary metastasis. Conclusions After microwave ablation treatment, the proliferating activity and invading capability of the remnant tumor cells are significantly decreased in a short period of time and their differentiation is improved after the treatment with microwave ablation. Though microwave ablation can not completely eradicate the tumor cells, it can prolong the survival time of the patients.
出处
《中华肝胆外科杂志》
CAS
CSCD
2005年第7期473-476,共4页
Chinese Journal of Hepatobiliary Surgery
关键词
肝癌
微波治疗
残留肿瘤
生物学特性
肿瘤生长
Carcinoma, hepatocellular
Microwave ablation
PCNA
MMP-9
Proliferation
Invasion
Metastasis
