摘要
目的:建立食药两用植物荨麻中槲皮苷含量的测定方法。方法:色谱柱:C18柱(150mm×4.6mm,5μm);流动相:甲醇-0.2%磷酸(体积比50:50);流速:1.0ml/min;柱温:室温;检测波长:360nm。结果:槲皮苷进样量在0.40～2.00μg范围内与峰面积线性关系良好(r=0.9996),回收率96.88%,RSD1.68%。结论:本法简便,结果可靠,可用于荨麻质量控制。
To establish a HPLC method for the determination of quercitrin in Urtica. Methods： The analysis was performed on C18 column （150μm × 416mm; 5 μm）, the mobile phase was MeOH-0.2% HsPO4 （50：50） with a flow rate at 1.0ml/min and wavelength at 360 nm under room temperature. Results： There was a good linear relationship when the sample size of quercitrin was at a range of 0.40-2.0μg （r=0.9996）, and the recovery was 96.88% with RSD at 1.68%： Conclusion： This method is simple, stable, fast, and reproducible and without the interference of impurity. It can be used for the content determination of quercitrin in Urtica.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2007年第1期288-290,共3页
Food Science
关键词
HPLC
荨麻
槲皮苷
含量测定
HPLC
urtica
quercitrin
content determination
