摘要
目的通过研究马尔尼菲青霉(PM)异柠檬酸裂解酶(ICL)基因转录水平与其酵母相生长的关系,初步探讨乙醛酸循环在PM致病中的意义。方法将PM分生孢子分别接种于中性培养基以及产生不同浓度一氧化氮的酸性培养基中,37℃培养72 h,采用实时定量RT-PCR法检测各组PM的ICL基因转录水平,检测340 nm吸光度评估PM的生长情况。结果不产一氧化氮的酸性培养基中PM较中性培养基中PM的ICL转录水平高(P<0.01),含菌量多(P<0.01);产一氧化氮的酸性培养基中PM的ICL转录水平降低(P<0.01),含菌量减少(P<0.01),且随一氧化氮浓度升高,下降趋势更明显,组间差异有显著性(P<0.01);ICL转录水平与生长之间呈正相关(相关系数r=0.99,P<0.01)。结论适当的酸性环境促进PM的ICL转录,利于PM的生长;一氧化氮明显抑制ICL转录,抑制PM的生长;乙醛酸循环可能在PM致病过程中起着重要的作用。
Objective To study the relationship between transcriptional level of isocitrate lyase and development of Penicillium marneffei yeast and to investigate the significance of glyoxylate pathway in the pathogenesis of P. mameffei. Methods P. mameffei was inoculated in acidic media and neutral media and was cultured at 37℃ for 72 h. Transcriptional levels of isocitrate lyase in groups of P. mameffei were detected by using real time RT - PCR. Development of P. mameffei was determined.by detecting A340. Results Transcriptional levels of isocitrate lyase and developmental levels in P. marneffei were higher in acidic media than those in nentral media. Nitric oxide inhibited transcription of isocitrate lyase and development of P. mameffei （P 〈 0.01 ）. Transcription of isocitrate lyase was correlated with development of P. mameffei, （r = 0. 99,P 〈 0. 01 ）. Conclusion Acidity can promote transcription of isocitrate lyase and development in P. mameffei ,which is inhibited by nitric oxide. The glyoxylate pathway may be important in the pathogenesis of P. mameffei.
出处
《广东医学》
CAS
CSCD
北大核心
2007年第2期185-187,共3页
Guangdong Medical Journal
基金
国家自然科学基金资助项目(编号:30470103)
