摘要
目的探讨氮气处理窒息死及缢死后大鼠心、肝、肺、肾中缺氧诱导因子1-α(HIF1-)α的表达变化规律及法医学意义。方法制作大鼠氮气处理窒息死及缢死两种窒息模型,记录其特征表现;应用免疫组织化学方法及图像分析技术测定大鼠窒息死后不同时间段HIF1-α在心肌、肺、肝、肾中的分布变化;结果HIF1-α表达于两种窒息模型的心肌细胞、肝细胞、肾小管细胞以及肺部各种细胞,各时间段的窒息组和对照组HIF1-α的表达有明显区别;氮气处理窒息死组四种组织在死亡后0、6和24h表达有减弱趋势,0h有核阳性表达,呼吸及心跳停止时间比缢死组短,各组织表达阳性率比缢死组稍弱;缢死组在除心肌表达逐渐增强外其他组织表达在6h出现一个表达高峰(此时核阳性表达最多),后表达逐渐减弱;对照组表达平稳,均未见到核阳性。结论在死亡后24h内检测组织中HIF1-α的表达可以作为推断窒息死的一个生物学指标。
Objective To investigate the expression of hypoxia-inducible factor 1-alpha （HIF1-α） in the heart, lung, liver and kidney in rats died of two typical models of asphyxia. Methods Two asphyxia models were made and tissue samples of the dead rats were collected from different groups at various postmortem duration. The expression and the changes of HIF1-α in various tissues were examined by immunohistochemistry and image analysis techniques. Results Significant expression of HIF1-α was observed in the myocardial fibers, kidney cells, liver cells and lung cells in both asphyxia models, but not in the control group. The expression of HIF1-α in various tissues in the rat died of nitrogen gas breathing was found in the nuclei at 0 hour and the expression level decreased gradually thereafter, The HIF1-α expression level and duration in various tissues of the rat died of hanging were higher and longer than that of the former group, with a peak of the expression level observed 6 hours after death, and then started to decline in all tissues except the heart where the expression still showed an increase 24 hours after death. The control groups showed a steady expression in the cytoplasm but not in the nuclei. Conclusion HIF1-α appears to be a valuable biomarker in the diagnosis of asphyxia within 24 hours after death.
出处
《法医学杂志》
CAS
CSCD
2007年第1期4-7,F0002,共5页
Journal of Forensic Medicine
基金
国家自然科学基金资助项目(30572091)
关键词
缺氧诱导因子1-Α
氮气处理窒息死
缢死
窒息
免疫组织化学
hypoxia inducible factor1-α
death by only breathing in nitrogen gas
death by hanging
asphyxia
immunohistochemistry
