摘要
剪股颖粒线虫[Anguina ag rostis(S teinbuch,1799)F ilipjev,1936]、小麦粒线虫[Anguina tritici(S teinbuch,1799)F ilipjev,1936]和维氏粒线虫[Anguina w evelli(Van den Berg,1985)S idd iq i,2000]都是重要的植物病原线虫,它们在成熟种子和虫瘿中的虫态通常是幼虫,而这3种线虫的幼虫形态非常相似,难以根据其特征对它们进行快速准确的种类鉴定。本研究根据这3种线虫的rDNA-ITS区域序列,分别设计筛选了特异性引物AgrF1/AgrR1、TriF1/TriR1、W evF1/W evR1,构建了这3种线虫单条幼虫多重PCR检测体系,获得3个大小差异明显的片段,表明这些引物设计合理,适合这3种线虫的快速准确检测。
Anguina agrostis, Anguina tritici and Anguina wevelli are important pests of agriculture. However, only juveniles are generally found in seed galls and similar in morphology. Three pairs of primers, named as AgrF1/AgrR1, TriF1/TriR1 and WevF1/WevR1, were designed based on rDNA-ITS nucleotide sequences of A. agrostis, A. tritici and A. wevelli. PCR techniques were developed and improved for detection of the three species with DNA extrated from single juvenile as templates. The amplified products for A. agrostis, A. tritici and A. wevelli were 499,617 and 377 bp in size and showed a strong unique band in electrophoresis respectively. This study indicated that the multiple-PCR technology could satisfy the need of detection of A. agrostis. A. tritici and A. wevelli.
出处
《植物病理学报》
CAS
CSCD
北大核心
2006年第6期508-511,共4页
Acta Phytopathologica Sinica
基金
国家食品安全关键技术专项课题(2001BA804A22)
