摘要
目的:从氧化损伤途径研究微波辐照引起组织血管通透性增高的原因。方法:实验于2003-03/07在解放军第三军医大学劳动卫生学教研室电磁辐射生物效应研究室完成。①动物实验:取健康雄性昆明种小鼠18只,单纯随机分为3组,即假辐照组,辐照后2,24h组,每组6只。假辐照组不进行辐照,辐照后2,24h组动物接受全身均匀30mW/cm2微波辐照20min,在辐照后2,24h,用荧光素纳示踪剂法测定微波暴露后小鼠主要脏器(脑、肺、肝、肾)血管通透性,同时用化学比色法测定组织丙二醛和超氧化物歧化酶含量。②细胞实验:血管内皮细胞株ECV304细胞生长至融合状态后接受30mW/cm220min微波辐照,在暴露后2,4,8,24h,用培养小室-白蛋白通透率法测定单层ECV304细胞对白蛋白的通透率,同时用化学比色法测定暴露前后细胞丙二醛和超氧化物歧化酶含量。以不接受微波辐照的ECV304细胞作为对照。结果:动物实验:①辐照后2h,脑、肺、肝、肾中荧光素纳含量分别较假辐照组高20.6%,23.8%,24.8%,20.2%(P均<0.05);辐照后24h,分别较假辐照组高31.2%,36.9%,38.2%,33.0%(P均<0.05)。②与假辐照组对比,暴露后2h组脑、肺、肝脏、肾脏组织超氧化物歧化酶含量分别低了13.3%,12.3%,13.6%,2.4%,丙二醛含量则分别高7.4%,20.9%,26.0%,12.3%;暴露后24h组超氧化物歧化酶含量分别低了19.5%,23.1%,20.5%,13.6%,丙二醛含量则分别高31.6%,41.1%,49.0%,16.6%。细胞实验:①在微波暴露后4,8,24h,ECV304细胞对biotin-BSA的通透率较对照分别高63.8%,96.6%,122%(P<0.05)。②辐照后2,4,8,24h,ECV304细胞超氧化物歧化酶水平分别比对照低了21.8%,29.1%,36.4%,52.1%(P均<0.05),丙二醛含量比对照高了12.5%,79.6%,87.5%,110%。结论:实验条件下微波辐照可引起受照组织或血管内皮细胞通透功能失衡,这与微波引起血管内皮细胞氧化还原平衡失调,造成内皮细胞氧化损伤有关。
AIM： To study the role of oxidative damage involved in the process of vascular permeability elevation in tissues caused by microwave irradiation. METHODS ： The experiment was conducted in the Electromagnetic Radiation Biological Effect Laboratory of the Third Military Medical University of Chinese PLA from March to July in 2003. ①Animal experiments： 18 healthy male KM mouse were divided into three groups, namely sham group and radiation group [ （parameters ware measured 2 hours post-irradiation） and group Ⅱ （parameters ware measured 24 hours post-irradiation）. All animals accepted 30 mW/cm^2 microwave irradiation on whole body for 20 minutes, and 2 hours or 24 hours post-irradiation later, vascular permeability of brain, lung, liver and kidney were determined by FINa tracer test, while malondialdehyde （MDA） and superoxide dismutase （SOD） in those tissues ware measured by chemical colorimetry contemporarily. ②Cell experiments： The cultured confluent endothelial cells ECV304 line ware exposed in 30 mW/cm^2 microwave field for 20 minutes and 2, 4, 8, 24 hours post-irradiation, percent of filtration of biotin-bovine serum albumin （BSA） on the monolayer ECV304 cells ware measured in Transwall cell culture chambers, meanwhile MDA and SOD ware measured by colorimetry. And ECV304 cells without irradiation ware taken as contols. RESULTS ： Animal experiment： ①At 2 hours post-irradiation, the FINa contents of brain, lung, liver and kidney were increased 20.6%, 23.8%, 24.8% and 20.2% respectively compared with those of sham group （P 〈 0.05）. And 24 hours after microwave irradiation, the content were increased 31.2%, 36.9%, 38.2%, 33.0% in radiation group （P 〈 0.05）. ② In comparison with sham group, the content of SOD in tissues of brain, lung, liver and kidney decreased 13.3%, 12.3%, 13.64%, 2.4% whereas MDA increased 7.4%, 20.9%, 26.0%, 12.3% respectively in radiation group Ⅰ （P 〈 0.05）. The contents of SOD ware declined 19.5%, 23.1%, 20.5%, 13.6% whereas those o
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2007年第1期103-106,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
国家自然科学基金资助项目(30270348)~~
