摘要
【目的】探讨临床验证有效的新羌防剂(由羌活、防风、荆芥穗等组成)抗流行性感冒(简称“流感”)的退热药效机制,对比超临界CO_(2)萃取工艺与水煎工艺新羌防剂的药效。【方法】建立流感病毒感染小鼠模型,脂多糖(LPS)、干酵母诱发发热大鼠模型,进行新羌防剂抗感染、解热的药效研究;测定荧光素钠浓度分析新羌防剂解热作用与加快肠系膜微循环的关系。【结果】新羌防剂显著改善流感病毒引发的肺部感染及LPS、干酵母感染诱发的动物发热症状,改善干酵母发热动物模型血氧浓度的降低,明显加速肠系膜对荧光素钠的吸收速度。【结论】新羌防剂加速肠系膜微循环对改善实验发热模型具有显著的量效关系。超临界CO_(2)萃取工艺的新羌防剂退热效果显著优于常规水煎工艺及已上市中成药和化药。
Objective To explore the mechanism of anti-influenza and antipyretic efficacy of the clinically proven effective Xinqiangfang Agent(composed of Notopterygii Rhizoma et Radix,Saposhnikoviae Radix,Schizonepetae Spica,etc.),and to conduct a comparative study on the efficacy of Xinqiangfang Agent of supercritical CO_(2) extraction process and water decoction process.Methods The anti-infective and antipyretic efficacy of Xinqiangfang Agent was investigated in a mouse model of influenza virus infection and a rat model of LPS or dried yeast induced fever;the relationship between the antipyretic effect of Xinqiangfang Agent and accelerated mesenteric microcirculation was explored by determination of fluorescein sodium(FINa).Results Xinqiangfang Agent significantly improved the symptoms of lung infection induced by influenza virus and fever induced by LPS or dry yeast infection in animals,improved the reduction of blood oxygen concentration in the dry yeast fever animal model,and significantly accelerate the effect of mesenteric absorption rate of sodium fluorescein.Conclusion The Xinqiangfang Agent has a significant quantity-effect relationship on improving the experimental fever model through accelerating mesenteric microcirculation.The antipyretic effect of Xinqiangfang Agent of supercritical CO_(2) extraction process was significantly superior to that of the conventional decoction process and the marketed proprietary Chinese medicines and chemical drugs.
作者
贾丹
张梓佳
冯毅凡
李卫民
周欣欣
JIA Dan;ZHANG Zi-Jia;FENG Yi-Fan;LI Wei-Min;ZHOU Xin-Xin(Guangzhou General Pharmaceutical Research Institute Co.,Ltd.(GPRI),Guangzhou 510000 Guangdong,China;Guangzhou University of Chinese Medicine,Guangzhou 510060 Guangdong,China;Guangdong Pharmaceutical University,Guangzhou 510060 Guangdong,China)
出处
《广州中医药大学学报》
CAS
2023年第9期2304-2313,共10页
Journal of Guangzhou University of Traditional Chinese Medicine
基金
2022年广州市科技计划重点项目(编号:SL2022B03J012)。
