摘要
灰树花是药食两用真菌,富含多糖、氨基酸和多种矿物元素。利用灰树花将无机锌转化为有机锌,能为人体补锌提供一条新的有效途径。稀土元素对生物的生长代谢具有促进作用。为了研究镧和锌元素存在时灰树花液体培养富集锌的能力,设计了四因素四水平的正交试验方案。其因素和水平分别为:硝酸镧浓度50、100、150、200(mg/L培养基),1L培养基中碳源马铃薯/葡萄糖150/18、200/24、250/30、300/36(g/g),氮源麸皮20、25、30、35(g/L培养基)和7水硫酸锌浓度100、600、1300、2000(mg/L培养基)。分别以干菌丝有机锌含量、25ml培养基有机锌产量和锌的转化率3个指标进行了分析,并分别给出了其最优培养条件,以用于指导灰树花液体培养基的设计。以25ml培养基有机锌产量为指标时,得到优化培养基为1L培养基中含ZnSO4·7H2O2000mg、麸皮35g、La(NO3)350mg、马铃薯300g、葡萄糖36g。优化培养基下有机锌的产量为1.472mg/25ml,即58.88mg/L培养基。
Grifolafrondosus is an edible and medicinal mushroom with rich polysaccharides ,amino acids and mineral elements. It is a new effective way to supply zinc to human body that Grifolafrondosus is used to transfer inorganic zinc to organic zinc what can be easily absorbed by human body. The rare earth element lanthanide has effect of promoting on the growth of Grifola frondosus. For studying the ability of Grifola frondosus to accumulate zinc when cultured in fiquid with the rare earth element lanthanide, authors designed a orthogonal test schemes which contained 4 factors and 4 levels. The factors and levels were as follows： La（NO3）3 concentration was 50, 100, 150, 200（mg/L culture medium）; potato/glucose 150/18, 200/24, 250/30, 300/36 （g/g, 1L culture medium）; bran 20, 25, 30, 35（g/L）; ZnSO4·7H2O concentration 100, 600, 1300, 2000（mg/L culture medium）. The authors have used four criteria to analyze the experiments results and have obtained the optimum culture conditions for zinc amount in dry biomaas, organic zinc yield and the zinc translation rate in order to guide technology design. With organic zinc yield in 25ml culture medium as criterion, the 1L optimal culture medium consists of ZnSO4·7H2O 2000rag, bran 35g, La（NO3）3 50mg, potato 300g, glucose 36g. The organic zinc yield in 25ml culture medium is 1.472mg/25ml, that is 58.88mg/L culture medium.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2006年第12期331-334,共4页
Food Science
基金
江苏社会发展科技计划资助项目(BS2002024)
关键词
灰树花
液体培养
锌
镧
Grifolafrondosus
liquid culture
Zinc
lanthanide
